Anti-CD41 antibody [EPR17876]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (AB181582)

Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD41 with ab181582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic staining on megakaryocytes and thrombocytes of rat spleen (PMID : 28133558). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Antigen Retrieval was heat mediated using 0.01M Tris-EDTA, pH 9.0 or 0.01M Sodium Citrate Buffer, pH 6.0.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (AB181582)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD41 with ab181582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic staining on megakaryocytes and thrombocytes of mouse spleen (PMID : 28133558). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.

Antigen Retrieval was heat mediated using 0.01M Tris-EDTA, pH 9.0 or 0.01M Sodium Citrate Buffer, pH 6.0.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-CD41 antibody [EPR17876] (AB181582)

Blocking/Dilution : 5% NFDM/TBST

Exposure : Lanes 1, 3, 4 : 3 minutes; lane 2 : 10 seconds; Lane 5 : 1 minute.

All lanes:

Western blot - Anti-CD41 antibody [EPR17876] (ab181582) at 1/1000 dilution

Lane 1:

Rat platelet lysate at 20 µg

Lane 2:

Mouse platelet lysate at 20 µg

Lane 3:

Mouse lung tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat spleen tissue lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 3 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 113 kDa

Observed band size: 113 kDa

true