Rabbit Monoclonal CD41 antibody. Suitable for Flow Cyt and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271), expandable thumbnail](https://content.abcam.com/products/images/cd41-antibody-epr27293-723-ab323271--flow-cytometry-img443718.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)")
![Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271), expandable thumbnail](https://content.abcam.com/products/images/cd41-antibody-epr27293-723-ab323271--flow-cytometry-img443719.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Human | Not recommended |
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
Alternative names
CD41, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb, Itga2b
Rabbit Monoclonal CD41 antibody. Suitable for Flow Cyt and reacts with Mouse samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Clone number
EPR27293-723
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271), expandable thumbnail](https://content.abcam.com/products/images/cd41-antibody-epr27293-723-ab323271--flow-cytometry-img443718.jpg "Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271)")
Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271)
Flow cytometric analysis of Purified mouse platelets (Upper Right, Lower Left and Right) from mouse blood (Upper Left) cells labeling CD41 with ab323271 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Platelets were isolated from mouse whole blood following the Abcam protocol "Isolation of Platelets from Whole Blood." https://www.abcam.com/en-us/technical-resources/protocols/isolation-of-human-platelets-from-whole-blood
Cells were co-stained with anti mouse CD61 conjugated to PE.![Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271), expandable thumbnail](https://content.abcam.com/products/images/cd41-antibody-epr27293-723-ab323271--flow-cytometry-img443719.jpg "Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271)")
Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (ab323271)
Flow cytometric analysis of Mouse blood cells labeling CD41 with ab323271 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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