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AB323271

Anti-CD41 antibody [EPR27293-723]

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Rabbit Recombinant Monoclonal CD41 antibody. Suitable for Flow Cyt and reacts with Mouse samples.

View Alternative Names

CD41, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb, Itga2b

2 Images
Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)

Flow cytometric analysis of Mouse blood cells labeling CD41 with ab323271 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD41 antibody [EPR27293-723] (AB323271)

Flow cytometric analysis of Purified mouse platelets (Upper Right, Lower Left and Right) from mouse blood (Upper Left) cells labeling CD41 with ab323271 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Platelets were isolated from mouse whole blood following the Abcam protocol "Isolation of Platelets from Whole Blood." https : //www.abcam.com/en-us/technical-resources/protocols/isolation-of-human-platelets-from-whole-blood
Cells were co-stained with anti mouse CD61 conjugated to PE.

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD41 antibody [EPR27293-723]

  • 578 PE

    PE Anti-CD41 antibody [EPR27293-723]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD41 antibody [EPR27293-723]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27293-723

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.
See full target information Itga2b
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com