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Anti-CD41 antibody [EPR4330] (ab134131) is a rabbit monoclonal antibody that is used to detect CD41 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Western blot - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Western blot - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% Tris glycine, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Expected
Expected
Tested

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

15 products for Alternative Product

1 product for Alternative Version

Target data

Function

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain (By similarity). Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen (PubMed:9111081). This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface (By similarity).

Alternative names

CD41, GP2B, ITGAB, ITGA2B, Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb

Recommended products

Anti-CD41 antibody [EPR4330] (ab134131) is a rabbit monoclonal antibody that is used to detect CD41 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4330
Purification technique
Affinity purification Protein A
Specificity

Mouse reactivity is only guaranteed for IHC-P.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-CD41 antibody [EPR4330] (ab134131) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse samples.

What is the molecular weight of CD41?


Anti-CD41 [EPR4330] (ab134131) specifically detects a band for CD41 (UniProt: P08514) at a molecular weight of 113kDa.

Recommended positive controls


Human platelet, fetal liver and placenta lysates; Human spleen tissue

Trusted by the scientific community


Anti-CD41 [EPR4330] (ab134131) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR4330] also available for your convenience:
ab134131, HRP - HRP Anti-CD41 antibody [EPR4330] ab194981, Carrier free - Anti-CD41 antibody [EPR4330] - Low endotoxin, Azide free ab229448, Carrier free - Anti-CD41 antibody [EPR4330] - BSA and Azide free ab271906, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-CD41 antibody [EPR4330] ab308537, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-CD41 antibody [EPR4330] ab311002, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-CD41 antibody [EPR4330] ab313040, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-CD41 antibody [EPR4330] ab313241, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-CD41 antibody [EPR4330] ab321077



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Negative control: K-562 (PMID 2458779)

    All lanes: Western blot - Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/mL

    Lane 1: HEL (human Erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 113 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.

    Negative control: K562( PMID 2458779)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

    All lanes: Western blot - Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution

    Lane 1: Human platelet lysate at 10 µg

    Lane 2: Human fetal liver lysate at 10 µg

    Lane 3: Human placenta lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 113 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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