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Anti-CD41 antibody [EPR4330] ab134131 is a rabbit monoclonal antibody that is used in CD41 western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR4330 is cited in over 40 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Western blot - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail
  • Western blot - Anti-CD41 antibody [EPR4330] (AB134131), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% Tris glycine, 40% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Expected
Expected
Tested

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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1 product for Alternative Version

Target data

Function

Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface.

Alternative names

Integrin alpha-IIb, GPalpha IIb, Platelet membrane glycoprotein IIb, GPIIb, ITGAB, ITGA2B, GP2B

Recommended products

Anti-CD41 antibody [EPR4330] ab134131 is a rabbit monoclonal antibody that is used in CD41 western blotting, IHC and immunofluorescence. Suitable for human and mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Antibody clone EPR4330 is cited in over 40 publications

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4330

Purification technique

Affinity purification Protein A

Specificity

Mouse reactivity is only guaranteed for IHC-P.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 in paraffin embedded Human bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/2000 dilution (0.30 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 27128503; PMID: 23667055).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

    Blocking and diluting buffer: 5% NFDM/TBST.

    Negative control: K-562 (PMID 2458779)

    All lanes: Western blot - Anti-CD41 antibody [EPR4330] (ab134131) at 0.6 µg/mL

    Lane 1: HEL (human Erythroleukemia erythroblast) whole cell lysate at 20 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 113 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 in HEL (human Erythroleukemia erythroblast) cells by ICC/IF (Immunocytochemistry/Immunofluorescence. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Samples were incubated with primary antibody at 1/100 dilution (6 μg/ml). An AlexaFluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 dilution (2 μg/ml). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) , Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 was used a counterstain antibody at 1/200 dilution (2.5 μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing membranous staining in HEL cell line.

    Negative control: K562( PMID 2458779)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR4330] (ab134131)

    ab134131 staining CD41 paraffin embedded Mouse bone marrow tissue sections by Immunohistochemistry. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody at 1/500 dilution (1.21 μg/ml). A ready to use Goat Anti-rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining in megakaryocytes of mouse bone marrow (PMID: 27128503).

  • Western blot - Anti-CD41 antibody [EPR4330] (ab134131), expandable thumbnail

    Western blot - Anti-CD41 antibody [EPR4330] (ab134131)

    All lanes: Western blot - Anti-CD41 antibody [EPR4330] (ab134131) at 1/1000 dilution

    Lane 1: Human platelet lysate at 10 µg

    Lane 2: Human fetal liver lysate at 10 µg

    Lane 3: Human placenta lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti rabbit at 1/2000 dilution

    Predicted band size: 113 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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