Rabbit Recombinant Monoclonal CD42a/GP-IX antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-CD42a/GP-IX antibody [EP5294] (AB133573), expandable thumbnail](https://content.abcam.com/products/images/cd42a-gp-ix-antibody-ep5294-ab133573--western-blot-img124360.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)")
![OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (AB133573), expandable thumbnail](https://content.abcam.com/products/images/cd42a-gp-ix-antibody-ep5294-ab133573--oi-rd-scanning-img68470.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000.00000 - 1/50000.00000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
Alternative names
CD42a, Platelet glycoprotein IX, GP-IX, GPIX, Glycoprotein 9, GP9
Recommended products
Rabbit Recombinant Monoclonal CD42a/GP-IX antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP5294
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 3.03 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-CD42a/GP-IX antibody [EP5294] (ab133573), expandable thumbnail](https://content.abcam.com/products/images/cd42a-gp-ix-antibody-ep5294-ab133573--western-blot-img124360.jpg "Western blot - Anti-CD42a/GP-IX antibody [EP5294] (ab133573)")
Western blot - Anti-CD42a/GP-IX antibody [EP5294] (ab133573)
All lanes: Western blot - Anti-CD42a/GP-IX antibody [EP5294] (ab133573) at 1/10000 dilution
Lane 1: Human platelet lysate at 10 µg
Lane 2: PBMC lysate at 10 µg
SecondaryAll lanes: Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
![OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (ab133573), expandable thumbnail](https://content.abcam.com/products/images/cd42a-gp-ix-antibody-ep5294-ab133573--oi-rd-scanning-img68470.jpg "OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (ab133573)")
OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (ab133573)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com