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AB133573

Anti-CD42a/GP-IX antibody [EP5294]

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(2 Publications)

Rabbit Recombinant Monoclonal CD42a/GP-IX antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD42a, Platelet glycoprotein IX, GP-IX, GPIX, Glycoprotein 9, GP9

2 Images
Western blot - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)
  • WB

Unknown

Western blot - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)

All lanes:

Western blot - Anti-CD42a/GP-IX antibody [EP5294] (ab133573) at 1/10000 dilution

Lane 1:

Human platelet lysate at 10 µg

Lane 2:

PBMC lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-CD42a/GP-IX antibody [EP5294] (AB133573)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-CD42a/GP-IX antibody [EP5294] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5294

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

The production method for this product has been changed from hybridoma to recombinant on 18th June 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The GPIb-V-IX complex functions as the vWF receptor and mediates vWF-dependent platelet adhesion to blood vessels. The adhesion of platelets to injured vascular surfaces in the arterial circulation is a critical initiating event in hemostasis. GP-IX may provide for membrane insertion and orientation of GP-Ib.
See full target information GP9
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 31:87 PubMed39237980

2024

Neuroprotective effects of intranasal extracellular vesicles from human platelet concentrates supernatants in traumatic brain injury and Parkinson's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Liling Delila,Ouada Nebie,Nhi Thao Ngoc Le,Kelly Timmerman,Deng-Yao Lee,Yu-Wen Wu,Ming-Li Chou,Luc Buée,Szu-Yi Chou,David Blum,David Devos,Thierry Burnouf

American journal of translational research 13:12461-12479 PubMed34956466

2021

CD105 expression is associated with invasive capacity in ovarian cancer and promotes invasiveness by inhibiting NDRG1 and regulating the epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Zhang,Xiubo Sang,Rui Zhang,Jingjing Chi,Wenpei Bai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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