AB233794

Anti-CD42b antibody [6H6H10]

Name: Anti-CD42b antibody [6H6H10] (ab233794) | Abcam
Brand: Abcam Limited
SKU: AB233794

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(1 Publication)

Mouse Monoclonal CD42b antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GP1BA aa 1-200.

View Alternative Names

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

5 Images

Flow Cytometry - Anti-CD42b antibody [6H6H10] (AB233794)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD42b antibody [6H6H10] (AB233794)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD42b antibody [6H6H10] (AB233794)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD42b with ab233794 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

Expected MW is 44.2 kDa.

All lanes:

Western blot - Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution

All lanes:

Human CD42b (aa 17-183) recombinant protein

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

All lanes:

Western blot - Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CD42b (aa 17-183)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 72 kDa

false

Supplier Data

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

All lanes:

Western blot - Anti-CD42b antibody [6H6H10] (ab233794) at 1/500 dilution

Lane 1:

HL-60 (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

MOLT-4 (human lymphoblastic leukemia cell line) cell lysate

Lane 3:

Ramos (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 72 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6H6H10

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human GP1BA aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P07359

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

See full target information GP1BA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Kidney international 81:458-68 PubMed22166849

2011

Functional metabotropic glutamate receptors 1 and 5 are expressed in murine podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Leyi Gu,Xinyue Liang,Lihua Wang,Yucheng Yan,Zhaohui Ni,Huili Dai,Jiayuan Gao,Shan Mou,Qin Wang,Xinyu Chen,Liming Wang,Jiaqi Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD42b antibody [6H6H10]

Flow Cytometry - Anti-CD42b antibody [6H6H10] (AB233794)

Flow Cytometry - Anti-CD42b antibody [6H6H10] (AB233794)

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

Western blot - Anti-CD42b antibody [6H6H10] (AB233794)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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Product promise