Mouse Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--flow-cytometry-img67310.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--immunocytochemistry-immunofluorescence-img78897.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--immunocytochemistry-immunofluorescence-img98561.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (AB252264)")
Publications
Filter by
- Biophysical journal 85:4099-1092003Kinetics of GPIbalpha-vWF-A1 tether bond under flow: effect of GPIbalpha mutations on the association and dissociation rates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesR Anand Kumar,Jing-fei Dong,Jenny A Thaggard,Miguel A Cruz,José A López,Larry V McIntirePubMed 14645097
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | IHC-Fr | ICC/IF | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Tested | Not recommended | Not recommended | Expected | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Version
5 products for Alternative Product
Target data
Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Alternative names
CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA
Recommended products
Mouse Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for Flow Cyt, IHC-Fr, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- AK2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab252264 is the carrier-free version of Anti-CD42b antibody [AK2] ab61402.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--flow-cytometry-img67310.jpg "Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)")
Flow Cytometry - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD42b with Anti-CD42b antibody [AK2] ab61402 at 1/490 (Right) compared with a Mouse IgG isotype control (Left). Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with mouse IgG (Left) or Anti-CD42b antibody [AK2] ab61402 (Right). Then stained with anti-CD41 conjugated to APC. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD42b antibody [AK2] ab61402).
![Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--immunocytochemistry-immunofluorescence-img78897.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)")
Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEL (human Erythroleukemia erythroblast) cells labelling CD42b with Anti-CD42b antibody [AK2] ab61402 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in HEL cells. Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: Anti-CD42b antibody [AK2] ab61402 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD42b antibody [AK2] ab61402).
![Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-ak2-bsa-and-azide-free-ab252264--immunocytochemistry-immunofluorescence-img98561.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)")
Immunocytochemistry/ Immunofluorescence - Anti-CD42b antibody [AK2] - BSA and Azide free (ab252264)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse splenocytes cells labelling CD42b with Anti-CD42b antibody [AK2] ab61402 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in mouse splenocytes. Anti-beta IV Tubulin antibody [EPR16775] ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/1000 dilution (Red). The Nuclear counterstain was DAPI (Blue).
The negative controls are as follows:
-ve control 1: Anti-CD42b antibody [AK2] ab61402 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080 AlexaFluor®594 Goat anti-Rabbit secondary secondary at 1/1000 dilution.
-ve control 2: Anti-beta IV Tubulin antibody [EPR16775] ab179504 at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1:1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD42b antibody [AK2] ab61402).
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