Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--western-blot-img27660.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61227.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53222.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)")
![Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunoprecipitation-img3518.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98265.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Alternative names
CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA
Recommended products
Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19204
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251517 is the carrier-free version of Anti-CD42b antibody [EPR19204] ab210407.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--western-blot-img27660.jpg "Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)")
Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)
This data was developed using Anti-CD42b antibody [EPR19204] ab210407, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD42b antibody [EPR19204] (Anti-CD42b antibody [EPR19204] ab210407) at 1/10000 dilution
Lane 1: Human fetal spleen tissue lysate at 10 µg
Lane 2: Human fetal brain tissue lysate at 10 µg
Lane 3: Human fetal heart tissue lysate at 10 µg
Lane 4: Human fetal kidney tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 120 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img61227.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)
This data was developed using Anti-CD42b antibody [EPR19204] ab210407, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD42b with Anti-CD42b antibody [EPR19204] ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on Human spleen platelets is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img53222.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)
This data was developed using Anti-CD42b antibody [EPR19204] ab210407, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human acute myelocytic leukemia tissue labeling CD42b with Anti-CD42b antibody [EPR19204] ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on megakaryocytes is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunoprecipitation-img3518.jpg "Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)")
Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)
This data was developed using Anti-CD42b antibody [EPR19204] ab210407, the same antibody clone in a different buffer formulation.CD42b was immunoprecipitated from 1mg of Human spleen whole cell lysate with Anti-CD42b antibody [EPR19204] ab210407 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-CD42b antibody [EPR19204] ab210407 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution. Lane 1: Human spleen whole cell lysate, 10µg (Input). Lane 2: Anti-CD42b antibody [EPR19204] ab210407 IP in Human spleen whole cell lysate. Lane 3: Rabbit IgG,monoclonal - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD42b antibody [EPR19204] ab210407 in Human spleen whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-CD42b antibody [EPR19204] (Anti-CD42b antibody [EPR19204] ab210407)
Predicted band size: 72 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr19204-bsa-and-azide-free-ab251517--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98265.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (ab251517)
This data was developed using Anti-CD42b antibody [EPR19204] ab210407, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CD42b with Anti-CD42b antibody [EPR19204] ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Membrane staining on lung cancer platelets is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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