Anti-CD42b antibody [EPR19204] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)

This data was developed using ab210407, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human acute myelocytic leukemia tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on megakaryocytes is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)

This data was developed using ab210407, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on Human spleen platelets is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)

This data was developed using ab210407, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling CD42b with ab210407 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Membrane staining on lung cancer platelets is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)

This data was developed using ab210407, the same antibody clone in a different buffer formulation.

CD42b was immunoprecipitated from 1mg of Human spleen whole cell lysate with ab210407 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab210407 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human spleen whole cell lysate, 10μg (Input).
Lane 2 : ab210407 IP in Human spleen whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal - Isotype Control (ab172730) instead of ab210407 in Human spleen whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-CD42b antibody [EPR19204] (<a href='/en-us/products/primary-antibodies/cd42b-antibody-epr19204-ab210407'>ab210407</a>)

Predicted band size: 72 kDa

false

• WB

Supplier Data

Western blot - Anti-CD42b antibody [EPR19204] - BSA and Azide free (AB251517)

This data was developed using ab210407, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD42b antibody [EPR19204] (<a href='/en-us/products/primary-antibodies/cd42b-antibody-epr19204-ab210407'>ab210407</a>) at 1/10000 dilution

Lane 1:

Human fetal spleen tissue lysate at 10 µg

Lane 2:

Human fetal brain tissue lysate at 10 µg

Lane 3:

Human fetal heart tissue lysate at 10 µg

Lane 4:

Human fetal kidney tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 72 kDa

Observed band size: 120 kDa

false

Exposure time: 3min