Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131436.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130185.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213)")
![OI-RD Scanning - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--oi-rd-scanning-img95850.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (AB221213)")
Publications
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- The Journal of infectious diseases 210:1700-102014Platelets direct monocyte differentiation into epithelioid-like multinucleated giant foam cells with suppressive capacity upon mycobacterial stimulation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
2 products for Alternative Version
Target data
Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Alternative names
CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA
Recommended products
Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR6995
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 4.7 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab221213 is the carrier-free version of Anti-CD42b antibody [EPR6995] ab134087.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131436.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)
Immunohistochemical analysis of paraffin embedded Human spleen tissue labelling CD42b with Anti-CD42b antibody [EPR6995] ab134087 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD42b antibody [EPR6995] ab134087).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130185.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)
Anti-CD42b antibody [EPR6995] ab134087 staining CD42b in human bone marrow tissue by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD42b antibody [EPR6995] ab134087).
![OI-RD Scanning - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-epr6995-low-endotoxin-azide-free-ab221213--oi-rd-scanning-img95850.jpg "OI-RD Scanning - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)")
OI-RD Scanning - Anti-CD42b antibody [EPR6995] - Low endotoxin, Azide free (ab221213)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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