Rabbit Recombinant Monoclonal CD42b antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Images
![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (AB227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-frozen-sections-img122054.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (AB227669)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136799.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669)")
![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (AB227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-frozen-sections-img41069.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (AB227669)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136752.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77561.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (AB227669)")
Publications
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- Nature nanotechnology 18:336-3422023Expansion-enhanced super-resolution radial fluctuations enable nanoscale molecular profiling of pathology specimens.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDominik Kylies,Marina Zimmermann,Fabian Haas,Maria Schwerk,Malte Kuehl,Michael Brehler,Jan Czogalla,Lola C Hernandez,Leonie Konczalla,Yusuke Okabayashi,Julia Menzel,Ilka Edenhofer,Sam Mezher,Hande Aypek,Bernhard Dumoulin,Hui Wu,Smilla Hofmann,Oliver Kretz,Nicola Wanner,Nicola M Tomas,Susanne Krasemann,Markus Glatzel,Christoph Kuppe,Rafael Kramann,Bella Banjanin,Rebekka K Schneider,Christopher Urbschat,Petra Arck,Nicola Gagliani,Marc van Zandvoort,Thorsten Wiech,Florian Grahammer,Pablo J Sáez,Milagros N Wong,Stefan Bonn,Tobias B Huber,Victor G PuellesPubMed 37037895
- Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association 30:1061272021Characterization of the 'White' Appearing Clots that Cause Acute Ischemic Stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOana Madalina Mereuta,Rosanna Rossi,Andrew Douglas,Sara Molina Gil,Seán Fitzgerald,Abhay Pandit,Ray McCarthy,Michael Gilvarry,Eric Ceder,Dennis Dunker,Annika Nordanstig,Petra Redfors,Katarina Jood,Georgios Magoufis,Klearchos Psychogios,Georgios Tsivgoulis,Alan O'Hare,Sarah Power,Paul Brennan,András Nagy,Ágnes Vadász,Waleed Brinjikji,David F Kallmes,Istvan Szikora,Alexandros Rentzos,Turgut Tatlisumak,John Thornton,Karen M DoylePubMed 34592611
- Journal of neurointerventional surgery 13:906-9112020High-resolution scanning electron microscopy for the analysis of three-dimensional ultrastructure of clots in acute ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOana Madalina Mereuta,Seán Fitzgerald,Trace A Christensen,Adam L Jaspersen,Daying Dai,Mehdi Abbasi,Tejaswini Puttappa,Ram Kadirvel,David F Kallmes,Karen M Doyle,Waleed BrinjikjiPubMed 33361274
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IHC-Fr | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
Target data
Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Alternative names
CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA
Recommended products
Rabbit Recombinant Monoclonal CD42b antibody. Suitable for IHC-P, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- SP202
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-frozen-sections-img122054.jpg "Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)
Immunohistochemistry (Frozen) analysis of rat spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136799.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)
Formalin-fixed, paraffin-embedded human spleen tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-frozen-sections-img41069.jpg "Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)
Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136752.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)
Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img77561.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)
Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136568.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)
Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669), expandable thumbnail](https://content.abcam.com/products/images/cd42b-antibody-sp202-ab227669--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136568.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)")
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