Name: Anti-CD42b antibody [SP219]
SKU: ab183345
Rating: 5 (7 reviews)

Anti-CD42b antibody [SP219] (ab183345) is a rabbit monoclonal antibody that is used to detect CD42b in Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr. Suitable for Human, Mouse samples.

- Specificity confirmed with CD42b knockout cell line validation

Related conjugates and formulations

ab240268
Carrier free
Anti-CD42b antibody [SP219] - BSA and Azide free
ab305653
Conjugated
PE Anti-CD42b antibody [SP219]
ab305655
Conjugated
HRP Anti-CD42b antibody [SP219]
ab308983
Conjugated
Alkaline Phosphatase Anti-CD42b antibody [SP219]
ab310755
Conjugated
Alexa Fluor® 594 Anti-CD42b antibody [SP219]
ab312777
Conjugated
Alexa Fluor® 568 Anti-CD42b antibody [SP219]
ab305654
Conjugated
APC Anti-CD42b antibody [SP219]
ab314946
Conjugated
Alexa Fluor® 647 Anti-CD42b antibody [SP219]
ab314271
Conjugated
Alexa Fluor® 488 Anti-CD42b antibody [SP219]
ab314255
Conjugated
Alexa Fluor® 555 Anti-CD42b antibody [SP219]
ab321275
Conjugated
Alexa Fluor® 750 Anti-CD42b antibody [SP219]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (AB183345), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	Flow Cyt (Intra)	IHC-Fr
Human	Tested	Tested	Expected
Mouse	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/20 - 1/100	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Species Human	Dilution info 1/20 - 1/100	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

8 products for Alternative Product

1 product for Alternative Version

Target data

See full target information GP1BA

Function

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

Alternative names

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: SP219
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-CD42b antibody [SP219] (ab183345) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), in Human, Mouse samples.

Recommended positive controls

IHC-P: Human spleen and bone marrow and mouse spleen tissues.IHC-Fr: Mouse spleen tissueFlow Cyt (intra): Mouse splenocytes cells, Jurkat and HEL cells.

Collaboration

Anti-CD42b [SP219] (ab183345) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trusted by the scientific community

Anti-CD42b [SP219] (ab183345) was first used in a scientific publication in 2014 and has been cited over 10 times in peer-reviewed journals.

Reviewed by scientists

Anti-CD42b [SP219] (ab183345) has over 5 independent reviews from customers.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed

The specificity of Anti-CD42b antibody [SP219] (ab183345) has been confirmed by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) testing in GP1BA Knockout Mouse Spleen samples.

Other related products

We have a range of other formats of antibody clone [SP219] also available for your convenience:
ab183345, Carrier free - ab240268, Carrier free - ab246709, ab279482, PE - ab305653, APC - ab305654, HRP - ab305655, Alkaline Phosphatase - ab308983, Alexa Fluor® 594 - ab310755, Alexa Fluor® 568 - ab312777, Alexa Fluor® 555 - ab314255, Alexa Fluor® 488 - ab314271, Alexa Fluor® 647 - ab314946, Alexa Fluor® 750 - ab321275

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets and megakaryocytes in the mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets in the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bone marrow tissue sections labeling CD42b with ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the megakaryocytes in the human bone marrow, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183345 for 10 mins at room temperature.
Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab183345 at 1/100 (1.9 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] (ab183345)
Flow cytometry analysis of mouse splenocytes cell labeling CD42b with purified ab183345 at 1/20 dilution (9.7 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control -Unlabeled cells (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD42b with ab183345 at 1/100 dilution.
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] (ab183345)
Flow cytometry analysis of HEL (human bone marrow erythroleukemia) labeling CD42b with purified ab183345 at 1/20 dilution (9.7 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black). Unlableled control - Unlabeled cells (blue).
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] (ab183345)
Flow cytometric analysis of rabbit anti-CD42b (SP219) antibody using ab183345 (diluted 1/400) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemical analysis of paraffin embedded human bone marrow tissue labeling CD42b with ab183345 at 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemical analysis of paraffin-embedded (A) Bone marrow tissue from wild-type C57BL/6JGpt mice (B) Bone marrow tissue from CD42b knockout mice staining with ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Bone marrow tissue from wild-type C57BL/6JGpt mice and no staining on (B) Bone marrow tissue from CD42b knockout mice. The section was incubated with ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] (ab183345)
Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice, (B) Spleen tissue from CD42b knockout mice staining with ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD42b knockout mice. The section was incubated with ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).