Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)

Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (AB240268), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IHC-Fr	Flow Cyt (Intra)
Human	Tested	Expected	Tested
Mouse	Tested	Tested	Expected
Dog	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Dog	Dilution info -	Notes -

Associated Products

Select an associated product type

11 products for Alternative Product

6 products for Alternative Version

Target data

See full target information GP1BA

Function

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

Alternative names

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

Recommended products

Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Mouse, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: SP219
Purification technique: Affinity purification Protein A/G
Concentration
Purification notes: Purified from TCS by protein A/G.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab240268 is the carrier-free version of Anti-CD42b antibody [SP219] ab183345.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling CD42b with Anti-CD42b antibody [SP219] ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets and megakaryocytes in the mouse spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD42b antibody [SP219] ab183345 for 10 mins at room temperature. This image was generated using Anti-CD42b antibody [SP219] ab183345, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD42b with Anti-CD42b antibody [SP219] ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on platelets in the human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD42b antibody [SP219] ab183345 for 10 mins at room temperature. This image was generated using Anti-CD42b antibody [SP219] ab183345, the same clone, but with a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bone marrow tissue sections labeling CD42b with Anti-CD42b antibody [SP219] ab183345 at 1/100 dilution (1.94 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the megakaryocytes in the human bone marrow, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with Anti-CD42b antibody [SP219] ab183345 for 10 mins at room temperature. This image was generated using Anti-CD42b antibody [SP219] ab183345, the same clone, but with a different buffer formulation.
Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified Anti-CD42b antibody [SP219] ab183345 at 1/100 (1.9 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Intracellular Flow Cytometry analysis of mouse splenocytes cell labeling CD42b with purified Anti-CD42b antibody [SP219] ab183345 at 1/20 dilution (9.7μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabeled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD42b with Anti-CD42b antibody [SP219] ab183345 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Intracellular Flow Cytometry analysis of HEL (human bone marrow erythroleukemia) labeling CD42b with purified Anti-CD42b antibody [SP219] ab183345 at 1/20 dilution (9.7μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black). Unlableled control - Unlabeled cells (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Intracellular flow cytometric analysis of rabbit anti-CD42b (SP219) antibody using Anti-CD42b antibody [SP219] ab183345 (diluted 1/400) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS,BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
Immunohistochemical analysis of paraffin embedded human bone marrow tissue labeling CD42b with Anti-CD42b antibody [SP219] ab183345 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-CD42b antibody [SP219] ab183345).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
This data was developed using Anti-CD42b antibody [SP219] ab183345, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Bone marrow tissue from wild-type C57BL/6JGpt mice (B) Bone marrow tissue from CD42b knockout mice staining with Anti-CD42b antibody [SP219] ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Bone marrow tissue from wild-type C57BL/6JGpt mice and no staining on (B) Bone marrow tissue from CD42b knockout mice. The section was incubated with Anti-CD42b antibody [SP219] ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - BSA and Azide free (ab240268)
This data was developed using Anti-CD42b antibody [SP219] ab183345, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) Spleen tissue from wild-type C57BL/6JGpt mice, (B) Spleen tissue from CD42b knockout mice staining with Anti-CD42b antibody [SP219] ab183345 at 1/500 dilution and ready-to-use Goat Anti-Rabbit IgG H&L (HRP) secondary. Counterstaining with hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. Positive staining on (A) Spleen tissue from wild-type C57BL/6JGpt mice and no staining on (B) Spleen tissue from CD42b knockout mice. The section was incubated with Anti-CD42b antibody [SP219] ab183345 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND™ RX instrument. The tissue samples were kindly provided by GemPharmatech. C57BL/6JGpt wildtype mice and CD42b-KO homozygous mice (Strain ID: T028631).