AB246709

Anti-CD42b antibody [SP219] - Low endotoxin, Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal CD42b antibody. Carrier free. Suitable for IHC-P, IHC-Fr, Flow Cyt (Intra) and reacts with Human, Mouse samples.

View Alternative Names

CD42b, Platelet glycoprotein Ib alpha chain, GP-Ib alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Antigen CD42b-alpha, GP1BA

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

This data was developed using ab183345, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded human spleen labelling CD42b with ab183345 at a concentration of 1 μg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. The heat-mediated antigen retrieval was conducted with at 100 °C, pH 8.5 for 32 mins. ab183345 Anti-CD42b antibody [SP219] was incubated for 16 mins, at 37 °C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control. Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Immunohistochemical analysis of paraffin embedded human spleen tissue labeling CD42b with ab183345 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Intracellular Flow Cytometry analysis of HEL (human bone marrow erythroleukemia) labeling CD42b with purified ab183345 at 1/20 dilution (9.7μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabeled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Intracellular flow cytometric analysis of rabbit anti-CD42b (SP219) antibody using ab183345 (diluted 1/400) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Immunohistochemical analysis of paraffin embedded human bone marrow tissue labeling CD42b with ab183345 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab183345 at 1/100 (1.9 μg/ml). Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD42b antibody [SP219] - Low endotoxin, Azide free (AB246709)

Intracellular Flow Cytometry analysis of mouse splenocytes cell labeling CD42b with purified ab183345 at 1/20 dilution (9.7μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control -Rabbit monoclonal IgG (ab172730) (Black). Unlableled control -Unlabeled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183345).

Carrier free

Anti-CD42b antibody [SP219] - BSA and Azide free
Unconjugated

Anti-CD42b antibody [SP219]
660 APC

APC Anti-CD42b antibody [SP219]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD42b antibody [SP219]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD42b antibody [SP219]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD42b antibody [SP219]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD42b antibody [SP219]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD42b antibody [SP219]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-CD42b antibody [SP219]
HRP

HRP Anti-CD42b antibody [SP219]
578 PE

PE Anti-CD42b antibody [SP219]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP219

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-Fr, Flow Cyt (Intra), IHC-P

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab246709 is the carrier-free version of ab183345.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G. Endotoxin level is less than 1 EU/ml as determined by the TAL test.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.

See full target information GP1BA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com