Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--flow-cytometry-img89761.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6968.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunocytochemistry-immunofluorescence-img21424.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121184.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (AB236548)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.
Alternative names
CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP
Recommended products
Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for Flow Cyt, WB, ICC/IF, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR21904
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236548 is the carrier-free version of Anti-CD43 antibody [EPR21904] ab235453.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--flow-cytometry-img89761.jpg "Flow Cytometry - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)")
Flow Cytometry - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)
Flow cytometric analysis of IM-9 (human multiple myeloma B lymphoblast cell line) cell line labeling CD43 with Anti-CD43 antibody [EPR21904] ab235453 at 1/50 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD43 antibody [EPR21904] ab235453).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img6968.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)
Immunohistochemical analysis of paraffin-embedded human B cell lymphoma tissue labeling CD43 with Anti-CD43 antibody [EPR21904] ab235453 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human B cell lymphoma (PMID: 2794085). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD43 antibody [EPR21904] ab235453).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunocytochemistry-immunofluorescence-img21424.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)")
Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized IM-9 (human multiple myeloma B lymphoblast cell line) cells labeling CD43 with Anti-CD43 antibody [EPR21904] ab235453 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on the IM-9 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD43 antibody [EPR21904] ab235453).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548), expandable thumbnail](https://content.abcam.com/products/images/cd43-antibody-epr21904-bsa-and-azide-free-ab236548--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121184.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR21904] - BSA and Azide free (ab236548)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD43 with Anti-CD43 antibody [EPR21904] ab235453 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in the T cell zone and scattered T cells in the germinal center (PMID: 2794085). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD43 antibody [EPR21904] ab235453).
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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