Anti-CD43 antibody [EPR28619-85] - BSA and Azide free

• mIHC

Lab

Multiplex immunohistochemistry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse colon tissue staining Glycam1 with ab324912 at a 1/4000 ( 0.126 μg/ml) dilution, ab317313 anti-CD43 used at 1/500 dilution (1.052 μg/ml) and ab272692 anti-MUC2 used at 1/2000 dilution (0.26 μg/ml).

Panel A : anti-Glycam1 (green; Opal™520), anti-CD43 (magenta; Opal™690), anti-MUC2 (gray; Opal™570) on mouse colon.

Panel B : anti-Glycam1 staining HEV (highly endothelial venules) in mouse colon.

Panel C : anti-CD43 staining T lymphocytes in mouse colon.

Panel D : anti-MUC2 staining goblet cells in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324912, ab237721 and ab315346 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse skeletal muscle.
The section was incubated with ab317313 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocyte cells labelling CD43 with ab317313 at 1/50 (10.52 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing membranous staining in subset of mouse splenocyte (shown in green), which is not co-localised with CD11b (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse spleen cell cells labelling CD43 with ab317313 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD4 conjugated to Alexa Fluor®647.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse spleen cell cells labelling CD43 with ab317313 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 510.
Gated on viable cells.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) EL4 (mouse lymphoma T lymphocyte). (B) C2C12 (mouse myoblast). tissue labeling CD43 with ab317313 at 1/100 (5.26 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) EL4, no staining on (B) C2C12.
The section was incubated with ab317313 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on immune cells of mouse lung.
The section was incubated with ab317313 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [EPR28619-85] - BSA and Azide free (AB317314)

This data was developed using ab317313, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD43 with ab317313 at 1/500 (1.052 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on T cells of mouse spleen.
The section was incubated with ab317313 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins