Anti-CD43 antibody [SP55]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101533 for 30 mins at room temperature.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab101533 for 30 mins at room temperature.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (AB101533)

Immunocytochemistry/ Immunofluorescence analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1 : 50 (2 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533)

Staining of Human CD43 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab101533 at a dilution of 1/200.

• Flow Cyt

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Flow Cytometry - Anti-CD43 antibody [SP55] (AB101533)

Flow Cytometry analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1 : 20 dilution (5.05 µg/ml) - Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD43 antibody [SP55] (AB101533)

Flow cytometric analysis of rabbit anti-CD43 (SP55) antibody ab101533 (1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

• WB

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Western blot - Anti-CD43 antibody [SP55] (AB101533)

All lanes:

Western blot - Anti-CD43 antibody [SP55] (ab101533) at 1/25 dilution

All lanes:

Jurkat cell lysate

Predicted band size: 39 kDa,40 kDa

Observed band size: 105 kDa,160 kDa,55 kDa

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