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Rabbit Recombinant Monoclonal CD43 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (AB101533), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (AB101533), expandable thumbnail
  • Flow Cytometry - Anti-CD43 antibody [SP55] (AB101533), expandable thumbnail
  • Flow Cytometry - Anti-CD43 antibody [SP55] (AB101533), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

Antigen retrieval is recommended: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.

Alternative names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

Recommended products

Rabbit Recombinant Monoclonal CD43 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP55
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101533 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab101533 for 30 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (ab101533)

    Immunocytochemistry/ Immunofluorescence analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1:50 (2 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)

    Flow Cytometry analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1:20 dilution (5.05 µg/ml) - Red. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue.

  • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)

    Flow cytometric analysis of rabbit anti-CD43 (SP55) antibody ab101533 (1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)

    Staining of Human CD43 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab101533 at a dilution of 1/200.

  • Western blot - Anti-CD43 antibody [SP55] (ab101533), expandable thumbnail

    Western blot - Anti-CD43 antibody [SP55] (ab101533)

    All lanes: Western blot - Anti-CD43 antibody [SP55] (ab101533) at 1/25 dilution

    All lanes: Jurkat cell lysate

    Predicted band size: 39 kDa, 40 kDa

    Observed band size: 105 kDa, 160 kDa, 55 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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