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Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (AB238799), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (AB238799), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] - BSA and Azide free (AB238799), expandable thumbnail
  • Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (AB238799), expandable thumbnail
  • Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (AB238799), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Antigen retrieval is recommended: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

Select an associated product type

15 products for Alternative Product

5 products for Alternative Version

Target data

Function

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.

Alternative names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

Recommended products

Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
SP55
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab238799 is the carrier-free version of Anti-CD43 antibody [SP55] ab101533.

What are the advantages of a recombinant monoclonal antibody?

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD43 with Anti-CD43 antibody [SP55] ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD43 antibody [SP55] ab101533 for 30 mins at room temperature. This image was generated using Anti-CD43 antibody [SP55] ab101533, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD43 with Anti-CD43 antibody [SP55] ab101533 at 1/200 dilution (0.55 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-CD43 antibody [SP55] ab101533 for 30 mins at room temperature. This image was generated using Anti-CD43 antibody [SP55] ab101533, the same clone, but with a different buffer formulation.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Immunocytochemistry/ Immunofluorescence analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified Anti-CD43 antibody [SP55] ab101533 at 1:50 (2 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238799)

  • Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Intracellular Flow Cytometry analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified Anti-CD43 antibody [SP55] ab101533 at 1/20 dilution (5.05 μg/ml) - Red. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) - Black. Unlabeled control - Blue. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238799)

  • Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Flow Cytometry - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Intracellular flow cytometric analysis of rabbit anti-CD43 (SP55) antibody Anti-CD43 antibody [SP55] ab101533 (1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD43 antibody [SP55] ab101533).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] - BSA and Azide free (ab238799)

    Staining of Human CD43 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using Anti-CD43 antibody [SP55] ab101533 at a dilution of 1/200.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-CD43 antibody [SP55] ab101533).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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