AB218523

Anti-CD43 antibody [SPN/1766R]

Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD43 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPN.

View Alternative Names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

4 Images

Supplier Data

Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (AB218523)

Immunocytochemistry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 μg/mL followed by Goat anti-Rabbit- IgG-CF488 (green). The nuclear counterstain is NucSpot (red).

Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (AB218523)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (AB218523)

Flow Cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 μg/10⁶ cells followed by Goat anti-Rabbit- IgG-CF488 (Blue); Isotype Control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (AB218523)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (AB218523)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD43 with ab218523 at 1 μg/ml.

Supplier Data

Western blot - Anti-CD43 antibody [SPN/1766R] (AB218523)

CD43 protein has multiple sites of glycosylation which increases the apparent molecular weight in Western blot.

All lanes:

Western blot - Anti-CD43 antibody [SPN/1766R] (ab218523) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SPN/1766R

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC, Flow Cyt, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SPN.

P16150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg/mL", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed : 18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed : 24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).. CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.

See full target information SPN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:2586 PubMed37142563

2023

Re-convolving the compositional landscape of primary and recurrent glioblastoma reveals prognostic and targetable tissue states.

Applications

Unspecified application

Species

Unspecified reactive species

Osama Al-Dalahmah,Michael G Argenziano,Adithya Kannan,Aayushi Mahajan,Julia Furnari,Fahad Paryani,Deborah Boyett,Akshay Save,Nelson Humala,Fatima Khan,Juncheng Li,Hong Lu,Yu Sun,John F Tuddenham,Alexander R Goldberg,Athanassios Dovas,Matei A Banu,Tejaswi Sudhakar,Erin Bush,Andrew B Lassman,Guy M McKhann,Brian J A Gill,Brett Youngerman,Michael B Sisti,Jeffrey N Bruce,Peter A Sims,Vilas Menon,Peter Canoll

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD43 antibody [SPN/1766R]

Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (AB218523)

Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (AB218523)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (AB218523)

Western blot - Anti-CD43 antibody [SPN/1766R] (AB218523)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise