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AB257327

Anti-CD43 antibody [SPN/2049R] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPN.

View Alternative Names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)

K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316 at 2 μg/ml, followed by Goat anti-Rabbit IgG CF488 (green) in ICC/IF.

The nuclear counter stain is NucSpot (red).

This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)

PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316, followed by Goat anti-Rabbit IgG CF488 (blue) as compared with an isotype control (red).

This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)
  • IHC-P

Ap

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)

Formalin-fixed, paraffin-embedded human tonsil tissue labeling CD43 using ab257316 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)
  • WB

Supplier Data

Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327)

This data was produced with ab257316, the same antibody in a different formulation with BSA and Azide.

All lanes:

Western blot - Anti-CD43 antibody [SPN/2049R] (<a href='/en-us/products/primary-antibodies/cd43-antibody-spn-2049r-ab257316'>ab257316</a>) at 2 µg/mL

All lanes:

K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SPN/2049R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, Flow Cyt, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human SPN.

P16150

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

ab257327 is the carrier-free version of ab257316.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed : 18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed : 24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity).. CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.
See full target information SPN
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:78 PubMed38554152

2024

Lipid droplets-related Perilipin-3: potential immune checkpoint and oncogene in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia He,Lingyun Liu,Yuexin Dong,Xiaoxin Zhang,Yuxian Song,Yue Jing,Yanhong Ni,Yi Wang,Zhiyong Wang,Liang Ding
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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