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Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPN.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327), expandable thumbnail
  • Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327), expandable thumbnail
  • Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (AB257327), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human SPN. Database link P16150

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
0.5-1 µg/mL
Notes

Incubate with primary antibody for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1-2 µg for 106 Cells
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1-2 µg/mL
Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Predominant cell surface sialoprotein of leukocytes which regulates multiple T-cell functions, including T-cell activation, proliferation, differentiation, trafficking and migration. Positively regulates T-cell trafficking to lymph-nodes via its association with ERM proteins (EZR, RDX and MSN) (By similarity). Negatively regulates Th2 cell differentiation and predisposes the differentiation of T-cells towards a Th1 lineage commitment. Promotes the expression of IFN-gamma by T-cells during T-cell receptor (TCR) activation of naive cells and induces the expression of IFN-gamma by CD4(+) T-cells and to a lesser extent by CD8(+) T-cells (PubMed:18036228). Plays a role in preparing T-cells for cytokine sensing and differentiation into effector cells by inducing the expression of cytokine receptors IFNGR and IL4R, promoting IFNGR and IL4R signaling and by mediating the clustering of IFNGR with TCR (PubMed:24328034). Acts as a major E-selectin ligand responsible for Th17 cell rolling on activated vasculature and recruitment during inflammation. Mediates Th17 cells, but not Th1 cells, adhesion to E-selectin. Acts as a T-cell counter-receptor for SIGLEC1 (By similarity). CD43 cytoplasmic tail. Protects cells from apoptotic signals, promoting cell survival.

Alternative names

CD43, SPN, Leukosialin, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Sialophorin, GALGP

Recommended products

Rabbit Recombinant Monoclonal CD43 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SPN.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human SPN. Database link P16150
Carrier free
Yes
Clone number
SPN/2049R
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from bioreactor concentrate.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

ab257327 is the carrier-free version of Anti-CD43 antibody [SPN/2049R] ab257316.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    Formalin-fixed, paraffin-embedded human tonsil tissue labeling CD43 using Anti-CD43 antibody [SPN/2049R] ab257316 at 2 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

    This data was produced with Anti-CD43 antibody [SPN/2049R] ab257316, the same antibody in a different formulation with BSA and Azide.

  • Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327), expandable thumbnail

    Flow Cytometry - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using Anti-CD43 antibody [SPN/2049R] ab257316, followed by Goat anti-Rabbit IgG CF488 (blue) as compared with an isotype control (red).

    This data was produced with Anti-CD43 antibody [SPN/2049R] ab257316, the same antibody in a different formulation with BSA and Azide.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using Anti-CD43 antibody [SPN/2049R] ab257316 at 2 μg/ml, followed by Goat anti-Rabbit IgG CF488 (green) in ICC/IF.

    The nuclear counter stain is NucSpot (red).

    This data was produced with Anti-CD43 antibody [SPN/2049R] ab257316, the same antibody in a different formulation with BSA and Azide.

  • Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327), expandable thumbnail

    Western blot - Anti-CD43 antibody [SPN/2049R] - BSA and Azide free (ab257327)

    This data was produced with Anti-CD43 antibody [SPN/2049R] ab257316, the same antibody in a different formulation with BSA and Azide.

    All lanes: Western blot - Anti-CD43 antibody [SPN/2049R] (Anti-CD43 antibody [SPN/2049R] ab257316) at 2 µg/mL

    All lanes: K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate

    Predicted band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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