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AB119348

Anti-CD44 antibody [1M7.8.1]

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(34 Publications)

Anti-CD44 antibody [1M7.8.1] (ab119348) is a rat monoclonal antibody detecting CD44 in ICC/IF. Suitable for Human, Mouse.

- Over 20 publications

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.

Confocal image showing strong membranous staining in HeLa cells.

Low expression control : MCF7 (PMID : 23039365).

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)

NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells.

  • Carrier free

    Anti-CD44 antibody [1M7.8.1] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

1M7.8.1

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-CD44 antibody [1M7.8.1] (ab119348) is a rat recombinant monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Trusted by the scientific community
Anti-CD44 [1M7.8.1] (ab119348) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Other related products
We have a range of other formats of antibody clone [1M7.8.1] also available for your convenience: ab119348, Carrier free - ab272229

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD44
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6590 PubMed40676021

2025

Near-infrared light-driven metabolic reprogramming of synoviocytes for the treatment of rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Hugang Zhang,Jiaxin Jia,Hanyu Liu,Haobo Han,Quanshun Li

Journal of nanobiotechnology 23:469 PubMed40598231

2025

Targeting YTHDF2 with pH-responsive siRNA nanoparticles suppresses MYC m6A modification and restores antitumor immunity in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziqi Guo,Qiuling Huang,Zhenzhen Cui,Cheng Yang,Liu Yang

The Journal of biological chemistry 301:110264 PubMed40409554

2025

The transcriptional regulator Sin3a activates CD44 and promotes collective luminal breast cancer cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Qiu,Guangxin Luan,Yiwen Liu,Yiqing He,Guoliang Zhang,Qian Guo,Cuixia Yang,Yan Du,Feng Gao

Cell death & disease 16:119 PubMed39979265

2025

Tubular CD44 plays a key role in aggravating AKI through NF-κB p65-mediated mitochondrial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jiewu Huang,Ping Meng,Ye Liang,Xiaolong Li,Shan Zhou,Jiemei Li,Xiaoxu Wang,Jinhua Miao,Weiwei Shen,Lili Zhou

Journal of inflammation research 17:10209-10222 PubMed39649425

2024

Comparative Proteomic Analysis of Endolymphatic Sac Luminal Fluid in Patients with Meniere's Disease and Controls.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwen Zhang,Qin Wang,Zhou Zhou,Anquan Peng,Wenqi Jiang

Journal of cellular physiology 240:e31482 PubMed39605294

2024

Spontaneously Immortalised Nonhuman Primate Müller Glia Cell Lines as Source to Explore Retinal Reprogramming Mechanisms for Cell Therapies.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Salman,Arantxa Bolinches-Amorós,Tina Storm,Daniela Moralli,Paulina Bryika,Angela J Russell,Stephen G Davies,Alun R Barnard,Robert E MacLaren

Journal of neuroinflammation 21:140 PubMed38807233

2024

Single-cell RNA sequencing reveals the evolution of the immune landscape during perihematomal edema progression after intracerebral hemorrhage.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Zhang,Cong Gao,Qiang Guo,Dongxu Yang,Guangning Zhang,Hao Lu,Liman Zhang,Guorong Zhang,Daojing Li

The FEBS journal 291:1719-1731 PubMed38275079

2024

Anchoring of hyaluronan glycocalyx to CD44 reduces sensitivity of HER2-positive gastric cancer cells to trastuzumab.

Applications

Unspecified application

Species

Unspecified reactive species

Si Chen,Guoliang Zhang,Yiwen Liu,Cuixia Yang,Yiqing He,Qian Guo,Yan Du,Feng Gao

Redox biology 68:102942 PubMed37918127

2023

Single-cell RNA sequencing of intestinal crypts reveals vital events in damage repair and the double-edged sword effect of the Wnt3/β-catenin pathway in irradiated mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tong Yuan,Junling Zhang,Yue Zhao,Yuying Guo,Saijun Fan

Bioengineering (Basel, Switzerland) 10: PubMed37106610

2023

Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System.

Applications

Unspecified application

Species

Unspecified reactive species

Didi Zha,Sagar Rayamajhi,Jared Sipes,Angela Russo,Harsh B Pathak,Kailiang Li,Mihaela E Sardiu,Leonidas E Bantis,Amrita Mitra,Rajni V Puri,Camille V Trinidad,Brian P Cain,Brett C Isenberg,Jonathan Coppeta,Shannon MacLaughlan,Andrew K Godwin,Joanna E Burdette
View all publications
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Product promise

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