Rat Recombinant Monoclonal CD44 antibody. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 23 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-ab119348--immunocytochemistry-immunofluorescence-img44065.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-ab119348--immunocytochemistry-immunofluorescence-img131424.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (AB119348)")
Publications
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- Bioengineering (Basel, Switzerland) 10:2023Proteomic Profiling of Fallopian Tube-Derived Extracellular Vesicles Using a Microfluidic Tissue-on-Chip System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDidi Zha,Sagar Rayamajhi,Jared Sipes,Angela Russo,Harsh B Pathak,Kailiang Li,Mihaela E Sardiu,Leonidas E Bantis,Amrita Mitra,Rajni V Puri,Camille V Trinidad,Brian P Cain,Brett C Isenberg,Jonathan Coppeta,Shannon MacLaughlan,Andrew K Godwin,Joanna E BurdettePubMed 37106610
- Cells 11:2022FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlena Kashirina,Alena Gavrina,Artem Mozherov,Dmitriy Kozlov,Daria Kuznetsova,Ekaterina Vorotelyak,Elena Zagaynova,Ekaterina Kalabusheva,Aleksandra KashinaPubMed 36078136
- Cell death & disease 13:2712022Mesenchymal stem cells protect against ferroptosis via exosome-mediated stabilization of SLC7A11 in acute liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFeiyan Lin,Wenyi Chen,Jiahang Zhou,Jiaqi Zhu,Qigu Yao,Bing Feng,Xudong Feng,Xiaowei Shi,Qiaoling Pan,Jiong Yu,Lanjuan Li,Hongcui CaoPubMed 35347117
- iScience 25:1036572022A Genetic modification that reduces ON-bipolar cells in hESC-derived retinas enhances functional integration after transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuguru Yamasaki,Hung-Ya Tu,Take Matsuyama,Matsuri Horiuchi,Tomoyo Hashiguchi,Junki Sho,Atsushi Kuwahara,Akiyoshi Kishino,Toru Kimura,Masayo Takahashi,Michiko MandaiPubMed 35024589
- Journal of materials chemistry. B 9:7205-72152021Hyaluronic acid drives mesenchymal stromal cell-derived extracellular matrix assembly by promoting fibronectin fibrillogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarisa Assunção,Chi Him Kendrick Yiu,Ho-Ying Wan,Dan Wang,Dai Fei Elmer Ker,Rocky S Tuan,Anna BlockiPubMed 33710248
- Communications biology 4:5272021Transcriptional heterogeneity of stemness phenotypes in the ovarian epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLauren E Carter,David P Cook,Curtis W McCloskey,Melanie A Grondin,David A Landry,Tiffany Dang,Olga Collins,Lisa F Gamwell,Holly A Dempster,Barbara C VanderhydenPubMed 33953351
- Cancer cell international 20:5632020CD44 cross-linking increases malignancy of breast cancer via upregulation of p-Moesin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Hu et. al.PubMed 33292278
- ACS nano 14:14655-146642020An Expanded Surface-Enhanced Raman Scattering Tags Library by Combinatorial Encapsulation of Reporter Molecules in Metal Nanoshells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSergio Rodal-Cedeira et. al.PubMed 32869970
- Experimental and therapeutic medicine 20:192020Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Huang et. al.PubMed 32934684
- Journal of Cancer 11:260-2712020CD44v6 Targeted by miR-193b-5p in the Coding Region Modulates the Migration and Invasion of Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Hu et. al.PubMed 31892992
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | ICC/IF | Flow Cyt | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/250 | Notes - |
Species Human | Dilution info 1/250 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Recommended products
Rat Recombinant Monoclonal CD44 antibody. Suitable for ICC/IF and reacts with Mouse, Human samples. Cited in 23 publications.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 1M7.8.1
- Purification technique
- Affinity purification Protein A
- Specificity
Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
This product has switched from a hybridoma to recombinant production method on 02nd November 2020
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-ab119348--immunocytochemistry-immunofluorescence-img44065.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells.![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-ab119348--immunocytochemistry-immunofluorescence-img131424.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] (ab119348)
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
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