Rat Recombinant Monoclonal CD44 antibody. Carrier free. Suitable for ICC/IF and reacts with Mouse, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (AB272229), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-bsa-and-azide-free-ab272229--immunocytochemistry-immunofluorescence-img73915.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (AB272229)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (AB272229), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-bsa-and-azide-free-ab272229--immunocytochemistry-immunofluorescence-img44066.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (AB272229)")
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Recommended products
Rat Recombinant Monoclonal CD44 antibody. Carrier free. Suitable for ICC/IF and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 1M7.8.1
- Purification technique
- Affinity purification Protein A
- Specificity
Detects a standard 85-kDa isoform of CD44 and a number of high molecular mass variant isoforms.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab272229 is the carrier-free version of Anti-CD44 antibody [1M7.8.1] ab119348.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-bsa-and-azide-free-ab272229--immunocytochemistry-immunofluorescence-img73915.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
This data was produced using the same clone but in a different formulation, Anti-CD44 antibody [1M7.8.1] ab119348.
HeLa (Human epithelial cell line from cervix adenocarcinoma) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-CD44 antibody [1M7.8.1] ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-CD44 antibody [1M7.8.1] ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in HeLa cells.
Low expression control: MCF7 (PMID: 23039365).
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-1m781-bsa-and-azide-free-ab272229--immunocytochemistry-immunofluorescence-img44066.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [1M7.8.1] - BSA and Azide free (ab272229)
This data was produced using the same clone but in a different formulation, Anti-CD44 antibody [1M7.8.1] ab119348.
NIH/3T3 (Mouse embryo fibroblast cell line) cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, Anti-CD44 antibody [1M7.8.1] ab119348 at 1/250 was incubated overnight at 4° C, followed by AlexaFluor® 488-conjugated Goat anti-Rat secondary antibody (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) at 1/1000 dilution at RT for 45 min. Anti-beta Tubulin antibody [EPR16774] ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with Anti-CD44 antibody [1M7.8.1] ab119348 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Confocal image showing strong membranous staining in NIH/3T3 cells.
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