AB157107

Anti-CD44 antibody

Name: Anti-CD44 antibody - mesenchymal stem cell marker (ab157107) | Abcam
Brand: Abcam Limited
SKU: AB157107
Price: 400 USD
Availability: InStock
Rating: 5 (31 reviews)

Lab Essentials
What is this?

(31 Reviews)

(248 Publications)

Anti-CD44 antibody (ab157107) is a rabbit polyclonal antibody detecting CD44 in Western Blot, IP, IHC-P. Suitable for Human, Mouse, Rat.

- Over 180 publications

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

9 Images

Supplier Data

Immunoprecipitation - Anti-CD44 antibody (AB157107)

Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines.

All lanes:

Immunoprecipitation - Anti-CD44 antibody (ab157107)

Predicted band size: 81 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (AB157107)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (AB157107)

Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human head and neck squamous cell carcinoma tissue staining CD-44 with ab157107 at 1 : 5000 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (AB157107)

Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human lung cancer tissue staining CD-44 with ab157107 at 1 : 5000 dilution.

Collaborator

Immunoprecipitation - Anti-CD44 antibody (AB157107)

Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with ab254530 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Immunoprecipitation - Anti-CD44 antibody (ab157107)

Predicted band size: 81 kDa

false

Supplier Data

Immunoprecipitation - Anti-CD44 antibody (AB157107)

Immunoprecipitatgion of HeLa cells with :

Lane 1 : Rabbit anti-CD44 recombinant monoclonal antibody

Lane 2 : ab157107 lot A303-872A-3

Lane 3 : ab157107 lot A303-872A-4

Lane 4 : Control IgG

All lanes:

Immunoprecipitation - Anti-CD44 antibody (ab157107)

Predicted band size: 81 kDa

false

Exposure time: 1s

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (AB157107)

Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of mouse plasmacytoma tissue staining CD-44 with ab157107 at 1 : 5000 dilution.

Collaborator

Western blot - Anti-CD44 antibody (AB157107)

ab157107 was shown to react with CD44 in wild-type HAP1 cells in Western blot with loss of signal observed in a CD44 knockout cell line. Wild-type HAP1 and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab157107 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-CD44 antibody (ab157107)

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-CD44 antibody (AB157107)

All lanes:

Western blot - Anti-CD44 antibody (ab157107) at 0.04 µg/mL

Lane 1:

A-549 (human lung carcinoma cell line) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 3:

U20S (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Predicted band size: 81 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-CD44 antibody (AB157107)

All lanes:

Western blot - Anti-CD44 antibody (ab157107) at 0.1 µg/mL

Lane 1:

TMCK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 81 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human CD44 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P16070

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Baboon": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Product Specifications

Anti-CD44 antibody (ab157107) is a rabbit polyclonal antibody and is validated for use in IHC-P, IP, WB in human, mouse, rat samples.
Anti-CD44 antibody (ab157107) specifically detects CD44 (UniProt ID: P16070; Molecular weight: 79kDa) and is sold in 100 µL selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CD44 antibody (ab157107) has high sensitivity and specificity.
Anti-CD44 antibody (ab157107) has been cited over 188 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD44 antibody (ab157107) has 31 independent reviews from customers.

Target Information

CD44 is a cell-surface glycoprotein involved in various physiological functions such as cell proliferation, adhesion, migration, and lymphocyte activation. It plays a significant role in the pathology of diseases like cancer, arthritis, and infections by interacting with its principal ligand, hyaluronic acid. CD44 plays a significant role in autoimmune diseases by mediating inflammatory responses and immune cell interactions. It is involved in the activation and migration of lymphocytes, which are crucial in the development of autoimmune conditions. For instance, targeting CD44 with monoclonal antibodies has shown anti-inflammatory effects in murine models of autoimmune diseases like arthritis and experimental autoimmune encephalomyelitis (EAE)

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.

See full target information CD44

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Publications (248)

Recent publications for all applications. Explore the full list and refine your search

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Experimental & molecular medicine 57:1963-1977 PubMed40887495

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Eicosapentaenoic acid restores inflammation-induced changes in chondrocyte mechanics by suppressing the NF-κB p65/CD44 signaling pathway and attenuates osteoarthritis.

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BMSC exosomes deliver JKAP to restore Th17/Treg balance via AKT/ERK, alleviating rheumatoid arthritis.

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Fang-Tian Xu,Yu Ling,Hui-Xian Wei,Lingzhang Meng,Dong Yin,Zhong-Hong Lai,Yu Huang,Xiao Huang,Hai-Ye Li,Qin-Wen Luo,Jian Song,Qiang Tang,Hong-Mian Li

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Effect of sulfated galactan with increased sulfation on cell migration and expression of cell adhesion molecules in sodium oxalate-induced HK-2 cell injury.

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Tawut Rudtanatip,Jenjiralai Phanphak,Somsuda Somintara,Jamal El-Abid,Kanokpan Wongprasert,José Kovensky,Waraporn Sakaew

Chemical biology & drug design 105:e70116 PubMed40346910

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Artesunate Enhances Sensitivity of Renal Cancer Cells to Sunitnib by Mediating Tripartite Motif Containing 24-Induced Ubiquitination of Paired Box 6.

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Puerarin relives inflammation, bone destruction and facilitates osteogenic differentiation in periodontitis by enhancing mitochondrial autophagy via activating mitochondrial Mitofusin 2.

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Yulan Xiang,Zelu Li,Xin He,Xiaoyang Chu,Chunyan Gao,Jiahao Guo,Yingyi Luan,Kai Yang,Dongliang Zhang

Discover oncology 16:627 PubMed40293538

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Spheroid morphology of lung cancer cell lines correlates with oncological profiles.

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Kaido Oishi,Keitaro Matsumoto,Shintaro Hashimoto,Fumitake Uchida,Ryosuke Hara,Masato Nishimuta,Takamune Matsumoto,Mayumi Iwatake,Koichi Tomoshige,Ryoichiro Doi,Ryusuke Machino,Tomohiro Obata,Takeshi Nagayasu

Signal transduction and targeted therapy 10:137 PubMed40274835

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Bio-barrier-adaptable biomimetic nanomedicines combined with ultrasound for enhanced cancer therapy.

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Juan Guo,Xueting Pan,Qingyuan Wu,Ping Li,Chaohui Wang,Shuang Liu,Haoyuan Zhang,Zezhong Huang,Xiaozhou Mou,Huiyu Liu,Jiajia Xue

International journal of molecular sciences 26: PubMed40243431

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Placental Molecular Expression of Different Pathogenic Vaginal Infections.

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Daniela Roxana Matasariu,Constantin Condac,Victoria Bîrluțiu,Ludmila Lozneanu,Iuliana Elena Bujor,Vasile Lucian Boiculese,Mihai Sava,Alexandra Ursache

Hereditas 162:60 PubMed40221814

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E2F1-driven EXOSC10 transcription promotes hepatocellular carcinoma growth and stemness: a potential therapeutic target.

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Haoyue Deng,Dingyong Wu,Yongpeng He,Xiaolei Yu,Jifei Liu,Yanrui Zhang,Bing Leng,Xiaofeng Yuan,Liguo Xiao

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Product promise

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