Anti-CD44 antibody is a rabbit polyclonal antibody that is used to detect CD44 in IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 185 publications
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- Cells 12:2023Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexey A Ivanov,Alla V Kuznetsova,Olga P Popova,Tamara I Danilova,Andrey V Latyshev,Oleg O YanushevichPubMed 37830549
- Breast cancer research : BCR 25:912023miR-203 drives breast cancer cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNuria G Martínez-Illescas,Silvia Leal,Patricia González,Osvaldo Graña-Castro,Juan José Muñoz-Oliveira,Alfonso Cortés-Peña,María Gómez-Gil,Zaira Vega,Verónica Neva,Andrea Romero,Miguel Quintela-Fandino,Eva Ciruelos,Consuelo Sanz,Sofía Aragón,Leisy Sotolongo,Sara Jiménez,Eduardo Caleiras,Francisca Mulero,Cristina Sánchez,Marcos Malumbres,María Salazar-RoaPubMed 37542268
- Journal of materials chemistry. B 11:5574-55852023Hybrid core-shell nanoparticles for cell-specific magnetic separation and photothermal heating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina de la Encarnación,Felix Jungwirth,David Vila-Liarte,Carlos Renero-Lecuna,Safiyye Kavak,Iñaki Orue,Claire Wilhelm,Sara Bals,Malou Henriksen-Lacey,Dorleta Jimenez de Aberasturi,Luis M Liz-MarzánPubMed 37040257
- Nature communications 14:27402023AMPK is a mechano-metabolic sensor linking cell adhesion and mitochondrial dynamics to Myosin-dependent cell migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Crosas-Molist,Vittoria Graziani,Oscar Maiques,Pahini Pandya,Joanne Monger,Remi Samain,Samantha L George,Saba Malik,Jerrine Salise,Valle Morales,Adrien Le Guennec,R Andrew Atkinson,Rosa M Marti,Xavier Matias-Guiu,Guillaume Charras,Maria R Conte,Alberto Elosegui-Artola,Mark Holt,Victoria Sanz-MorenoPubMed 37217519
- Oncology letters 25:2072023Increased soluble E‑cadherin of spheroid formation supplemented with fetal bovine serum in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIn-Youb Chang,Sang-Pil YoonPubMed 37123020
- EMBO reports 24:e565242023Pericyte stem cells induce Ly6G cell accumulation and immunotherapy resistance in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhichong Wu,Kevin Thierry,Sophie Bachy,Xinyi Zhang,Pia Gamradt,Hector Hernandez-Vargas,Ivan Mikaelian,Laurie Tonon,Roxanne Pommier,Yajie Zhao,Philippe Bertolino,Ana HenninoPubMed 36802267
- Frontiers in molecular biosciences 10:10268102023Proteolysis of CD44 at the cell surface controls a downstream protease network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBirte Wöhner,Wenjia Li,Sven Hey,Alice Drobny,Ludwig Werny,Christoph Becker-Pauly,Ralph Lucius,Friederike Zunke,Stefan Linder,Philipp ArnoldPubMed 36876041
- International journal of molecular sciences 24:2023Nanomechanical Signatures in Glioma Cells Depend on CD44 Distribution in IDH1 Wild-Type but Not in IDH1R132H Mutant Early-Passage Cultures.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMikhail E Shmelev,Vladislav M Farniev,Nikita A Shved,Vadim V KumeikoPubMed 36835465
- Bioactive materials 25:256-2722023"Slow walk" mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Sun,Yau Tsz Chan,Ki Wai Kevin Ho,Li Zhang,Liming Bian,Rocky S Tuan,Yangzi JiangPubMed 36825224
- Frontiers in cellular neuroscience 16:10968722023Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDorota Piekna-Przybylska,Daxiang Na,Jingyuan Zhang,Cameron Baker,John M Ashton,Patricia M WhitePubMed 36687526
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD44 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16070
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Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Tested | Expected | Expected |
| Baboon | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Guinea pig | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Platypus | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Human | Dilution info 1/500 - 1/2000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-10 µg/mg of lysate | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 - 1/10000 | Notes - |
Species Human | Dilution info 1/2000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Platypus | Dilution info - | Notes - |
Associated Products
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Target data
Function
The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Recommended products
Anti-CD44 antibody is a rabbit polyclonal antibody that is used to detect CD44 in IHC-P, IP, Western blot. Suitable for Human, Mouse, Rat samples.
- Cited in over 185 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD44 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16070
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab157107 was affinity purified using an epitope specific to CD44 immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Product Specifications
Anti-CD44 antibody (ab157107) is a rabbit polyclonal antibody and is validated for use in IHC-P, IP, WB in human, mouse, rat samples.
Anti-CD44 antibody (ab157107) specifically detects CD44 (UniProt ID: P16070; Molecular weight: 79kDa) and is sold in 100 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-CD44 antibody (ab157107) has high sensitivity and specificity.
Anti-CD44 antibody (ab157107) has been cited over 188 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD44 antibody (ab157107) has 31 independent reviews from customers.
Target Information
CD44 is a cell-surface glycoprotein involved in various physiological functions such as cell proliferation, adhesion, migration, and lymphocyte activation. It plays a significant role in the pathology of diseases like cancer, arthritis, and infections by interacting with its principal ligand, hyaluronic acid. CD44 plays a significant role in autoimmune diseases by mediating inflammatory responses and immune cell interactions. It is involved in the activation and migration of lymphocytes, which are crucial in the development of autoimmune conditions. For instance, targeting CD44 with monoclonal antibodies has shown anti-inflammatory effects in murine models of autoimmune diseases like arthritis and experimental autoimmune encephalomyelitis (EAE)
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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9 product images
Immunoprecipitation - Anti-CD44 antibody (ab157107)
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with Anti-CD44 antibody [Hermes-3] ab254530 at 1/2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines.
All lanes: Immunoprecipitation - Anti-CD44 antibody (ab157107)
Predicted band size: 81 kDa
Immunoprecipitation - Anti-CD44 antibody (ab157107)
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab157107 pre-coupled to prot.A-Sepharose beads. Samples were washed and processed for western blot with Anti-CD44 antibody [Hermes-3] ab254530 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Immunoprecipitation - Anti-CD44 antibody (ab157107)
Predicted band size: 81 kDa
Immunoprecipitation - Anti-CD44 antibody (ab157107)
Immunoprecipitatgion of HeLa cells with:
Lane 1: Rabbit anti-CD44 recombinant monoclonal antibody
Lane 2: ab157107 lot A303-872A-3
Lane 3: ab157107 lot A303-872A-4
Lane 4: Control IgG
All lanes: Immunoprecipitation - Anti-CD44 antibody (ab157107)
Predicted band size: 81 kDa
Exposure time: 1s
Western blot - Anti-CD44 antibody (ab157107)
ab157107 was shown to react with CD44 in wild-type HAP1 cells in Western blot with loss of signal observed in a CD44 knockout cell line. Wild-type HAP1 and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab157107 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-CD44 antibody (ab157107)
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of mouse plasmacytoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human lung cancer tissue staining CD-44 with ab157107 at 1:5000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody (ab157107)
Immunohistochemistry analysis (formalin-fixed, paraffin embedded sections) of human head and neck squamous cell carcinoma tissue staining CD-44 with ab157107 at 1:5000 dilution.
Western blot - Anti-CD44 antibody (ab157107)
All lanes: Western blot - Anti-CD44 antibody (ab157107) at 0.1 µg/mL
Lane 1: TMCK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 2: NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 30s
Western blot - Anti-CD44 antibody (ab157107)
All lanes: Western blot - Anti-CD44 antibody (ab157107) at 0.04 µg/mL
Lane 1: A-549 (human lung carcinoma cell line) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 3: U20S (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Predicted band size: 81 kDa
Exposure time: 30s
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