AB24504

Anti-CD44 antibody

(3 Reviews)

(35 Publications)

Rabbit Polyclonal CD44 antibody. Suitable for WB and reacts with Mouse samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human CD44.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images

Supplier Data

Western blot - Anti-CD44 antibody (AB24504)

All lanes:

Western blot - Anti-CD44 antibody (ab24504)

All lanes:

Mouse small intestine tissue lysate.

Predicted band size: 81 kDa

false

CiteAb

Western blot - Anti-CD44 antibody (AB24504)

CD44 western blot using anti-CD44 antibody ab24504. Publication image and figure legend from Cho, S., Roh, K., et al., 2017, Sci Rep, PubMed 28232732.

ab24504 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab24504 please see the product overview.

Subcutaneous hyaluronidase injection alters cytokine expression. Analysis of cytokine expression in total mouse leg tissue. (A) Quantitative real-time PCR analysis of IL-6 mRNA expression. Data are presented as means ± SEMs. (B) ELISA analysis of the levels of TGF-β, IL-4, IFN-γ, and IL-12. Data are expressed as median optical intensities. Data are presented as means ± SEMs. (C) Representative western blot of IFN-γ and CD44 expression. **p < 0.01, ***p < 0.001.

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human CD44. The exact immunogen used to generate this antibody is proprietary information.

P16070

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-4 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

This product is manufactured by BioVision, an Abcam company and was previously called 3393 HCAM Antibody. 3393-100 is the same size as the 100 µg size of ab24504.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Thimerosal (merthiolate) Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.

See full target information CD44

style

left-column

Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 16: PubMed39458645

2024

Priming Mesenchymal Stem Cells with Lipopolysaccharide Boosts the Immunomodulatory and Regenerative Activity of Secreted Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Aina Areny-Balagueró,Marta Camprubí-Rimblas,Elena Campaña-Duel,Anna Solé-Porta,Adrián Ceccato,Anna Roig,John G Laffey,Daniel Closa,Antonio Artigas

International journal of nanomedicine 16:8087-8102 PubMed34934314

2021

Regulatory Effects of Damaged Renal Epithelial Cells After Repair by Polysaccharides with Different Sulfation Degree on the Calcium Oxalate Crystal-Cell Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Xin-Yuan Sun,Hui Zhang,Ji-Wang Deng,Bang-Xian Yu,Yi-Han Zhang,Jian-Ming Ouyang

Clinical and translational medicine 11:e558 PubMed34841686

2021

Epicardial and endothelial cell activation concurs with extracellular matrix remodeling in atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Nicoline W E van den Berg,Makiri Kawasaki,Benedetta Fabrizi,Fransisca A Nariswari,Arianne C Verduijn,Jolien Neefs,Robin Wesselink,Rushd F M Al-Shama,Allard C van der Wal,Onno J de Boer,Jan Aten,Antoine H G Driessen,Aldo Jongejan,Joris R de Groot

American journal of cancer research 11:3475-3495 PubMed34354856

2021

Microenvironment of mammary fat pads affected the characteristics of the tumors derived from the induced cancer stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hagar A Abu Quora,Maram H Zahra,Samah El-Ghlban,Neha Nair,Said M Afify,Ghmkin Hassan,Hend M Nawara,Mona Sheta,Sadia Monzur,Xiaoying Fu,Amira Osman,Akimasa Seno,Masaharu Seno

American journal of cancer research 11:441-457 PubMed33575080

2021

Isolation and characterization of cancer stem cells derived from human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroko Ishii,Yuki Mimura,Maram H Zahra,Shota Katayama,Ghmkin Hassan,Said M Afify,Masaharu Seno

Cellular & molecular immunology 18:711-722 PubMed32728200

2020

The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33.

Applications

Unspecified application

Species

Unspecified reactive species

M Liguori,E Digifico,A Vacchini,R Avigni,F S Colombo,E M Borroni,F M Farina,S Milanesi,A Castagna,L Mannarino,I Craparotta,S Marchini,E Erba,N Panini,M Tamborini,V Rimoldi,P Allavena,C Belgiovine

Cells 9: PubMed32751857

2020

Alveolar Type II Cells or Mesenchymal Stem Cells: Comparison of Two Different Cell Therapies for the Treatment of Acute Lung Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Guillamat-Prats,Marta Camprubí-Rimblas,Ferranda Puig,Raquel Herrero,Neus Tantinyà,Anna Serrano-Mollar,Antonio Artigas

Cancer research 80:444-457 PubMed31722999

2019

CD44 Promotes PD-L1 Expression and Its Tumor-Intrinsic Function in Breast and Lung Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Kong,Ryuhjin Ahn,Kangning Yang,Xianbing Zhu,Zheng Fu,Geneviève Morin,Rachel Bramley,Nikki C Cliffe,Yibo Xue,Hellen Kuasne,Qinghao Li,Sungmi Jung,Anne V Gonzalez,Sophie Camilleri-Broet,Marie-Christine Guiot,Morag Park,Josie Ursini-Siegel,Sidong Huang

Bioengineering (Basel, Switzerland) 6: PubMed31450740

2019

Metastasis of Cancer Stem Cells Developed in the Microenvironment of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Said M Afify,Ghmkin Hassan,Amira Osman,Anna Sanchez Calle,Hend M Nawara,Maram Hussein Zahra,Samah El-Ghlban,Hager Mansour,Md Jahangir Alam,Hagar A Abu Quora,Juan Du,Akimasa Seno,Yoshiaki Iwasaki,Masaharu Seno

Frontiers in cardiovascular medicine 6:81 PubMed31275946

2019

and Approaches Reveal Novel Insight Into the Ability of Epicardium-Derived Cells to Create Their Own Extracellular Environment.

Applications

Unspecified application

Species

Unspecified reactive species

Noortje A M Bax,Sjoerd N Duim,Boudewijn P T Kruithof,Anke M Smits,Carlijn V C Bouten,Marie José Goumans

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB189524

Anti-CD44 antibody [EPR18668]

RabMAb|Recombinant|Lab Essentials|20ul selling size|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR18668] (AB189524)

Primary Antibodies

AB216647

Anti-CD44 antibody [EPR1013Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647)

Primary Antibodies

AB232556

Anti-CD44 antibody [EPR18668] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR18668] - BSA and Azide free (AB232556)

Primary Antibodies

AB51037

Anti-CD44 antibody [EPR1013Y]

RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] (AB51037)

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com