Anti-CD44 antibody [BLR038F]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (AB243894)

Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded breast carcinoma labeling human CD44 with ab243894, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Membrane staining on breast carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

• ICC

Unknown

Immunocytochemistry - Anti-CD44 antibody [BLR038F] (AB243894)

Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling CD44 using ab243894 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (AB243894)

Formalin-fixed, paraffin-embedded human head and neck squamous cell carcinoma tissue stained for CD44 using ab243894 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.

• Flow Cyt

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Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894)

Flow Cytometry analysis of HeLa cells labeling CD44 with ab243894 (red). Cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Isotype control (unshaded). Secondary : DyLight^® 650-conjugated goat anti-rabbit IgG.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (AB243894)

Immunohistochemical (IHC-IF) analysis of Formalin-Fixed Paraffin-Embedded head and neck squamous cell carcinoma tissue labeling human CD44 (Orange) with ab243894 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : Opal™. Membrane staining on head and neck squamous cell carcinoma tissue is observed. Counter stained with DAPI (Blue). Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

• Flow Cyt

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Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894)

Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

• ICC

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Immunocytochemistry - Anti-CD44 antibody [BLR038F] (AB243894)

Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded 786-O cells labeling human CD44 with ab243894 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate : DAB. Membrane staining on786-O cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.

• IP

Unknown

Immunoprecipitation - Anti-CD44 antibody [BLR038F] (AB243894)

CD44 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243894 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243894 at 1/1000 dilution. Lane 1 : ab243894 IP in HeLa whole cell lysate.

Lane 2 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)

Predicted band size: 81 kDa

false

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894)

Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : Goat Anti-Rabbit IgG H&L (DyLight® 650) (ab96886).

• WB

Unknown

Western blot - Anti-CD44 antibody [BLR038F] (AB243894)

Detection : chemiluminescence with an exposure time of 3 seconds.

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

786-O whole cell lysate at 50 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

Jurkat-3 whole cell lysate at 50 µg

Lane 5:

A549 whole cell lysate at 50 µg

Lane 6:

MCF7 whole cell lysate at 50 µg

Lane 7:

HepG2 whole cell lysate at 50 µg

Lane 8:

KG-1 whole cell lysate at 50 µg

Lane 9:

RPMI 8226 whole cell lysate at 50 µg

Lane 10:

MOLT-4 whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG antibody

Predicted band size: 81 kDa

false

• WB

Lab

Western blot - Anti-CD44 antibody [BLR038F] (AB243894)

Lanes 1 - 4 : Merged signal (red and green). Green - ab243894 observed at 80 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab243894 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab243894 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

CD44 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CD44 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-cd44-knockout-hela-cell-line-ab262515'>ab262515</a>)

Lane 3:

A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

• WB

Supplier Data

Western blot - Anti-CD44 antibody [BLR038F] (AB243894)

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 10 µg

Lane 2:

786-O whole cell lysate at 10 µg

Lane 3:

HEK293T whole cell lysate at 10 µg

Lane 4:

A-549 whole cell lysate at 10 µg

Lane 5:

KG-1 cells whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 81 kDa

Observed band size: 90 kDa

true

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-CD44 antibody [BLR038F] (AB243894)

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 10 µg

Lane 2:

CT26 whole cell lysate at 10 µg

Lane 3:

CH27 whole cell lysate at 10 µg

Lane 4:

TCMK-1 whole cell lysate at 10 µg

Lane 5:

BW5147.3 whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 85 kDa

Observed band size: 90 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-CD44 antibody [BLR038F] (AB243894)

Detection method : Chemiluminescence

All lanes:

Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution

Lane 1:

BW5147.3 cell lysate at 50 µg

Lane 2:

EL4 cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 81 kDa

false

Exposure time: 10s