Anti-CD44 antibody [BLR038F] KO tested (ab243894)

Rabbit Recombinant Monoclonal CD44 antibody. Suitable for IHC-P, ICC, IP, Flow Cyt, WB and reacts with Human, Mouse samples. Cited in 14 publications. Immunogen corresponding to Synthetic Peptide within Human CD44 aa 700 to C-terminus.

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Images

Flow Cytometry - Anti-CD44 antibody [BLR038F] (AB243894), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human CD44 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16070

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	ICC	IP	Flow Cyt	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Expected	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100.00000 - 1/500.00000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Use 20μl/mg lysate.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1 µL for 10⁶ Cells	Notes -
Species Mouse	Dilution info 1 µL for 10⁶ Cells	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Associated Products

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Target data

See full target information CD44

Function

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Alternative names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human CD44 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P16070
Clone number: BLR038F
Concentration
Purification notes: Recombinant antibody was purified from cell culture supernatant.

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is sold under License from Bethyl Laboratories, Inc.

Product promise

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16 product images

Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)
Flow Cytometry analysis of HeLa cells labeling CD44 with ab243894 (red). Cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Isotype control (unshaded). Secondary: DyLight^® 650-conjugated goat anti-rabbit IgG.
Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)
CD44 was immunoprecipitated from 1.0 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243894 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243894 at 1/1000 dilution. Lane 1: ab243894 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
All lanes: Immunoprecipitation - Anti-CD44 antibody [BLR038F] (ab243894)
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)
Formalin-fixed, paraffin-embedded human head and neck squamous cell carcinoma tissue stained for CD44 using ab243894 at 1/500 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)
Formalin-fixed, paraffin-embedded HCT 116 (human colorectal carcinoma cell line) cells labeling CD44 using ab243894 at 1/500 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)
Lanes 1 - 4: Merged signal (red and green). Green - ab243894 observed at 80 kDa. Red - loading control, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab243894 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab243894 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: CD44 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2: Western blot - Human CD44 knockout HeLa cell line (Human CD44 knockout HeLa cell line ab262515)
Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4: LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 80 kDa
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)
Detection: chemiluminescence with an exposure time of 3 seconds.
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 786-O whole cell lysate at 50 µg
Lane 3: HEK-293T whole cell lysate at 50 µg
Lane 4: Jurkat-3 whole cell lysate at 50 µg
Lane 5: A549 whole cell lysate at 50 µg
Lane 6: MCF7 whole cell lysate at 50 µg
Lane 7: HepG2 whole cell lysate at 50 µg
Lane 8: KG-1 whole cell lysate at 50 µg
Lane 9: RPMI 8226 whole cell lysate at 50 µg
Lane 10: MOLT-4 whole cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG antibody
Predicted band size: 81 kDa
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)
Detection method: Chemiluminescence
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1: BW5147.3 cell lysate at 50 µg
Lane 2: EL4 cell lysate at 50 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Predicted band size: 81 kDa
Exposure time: 10s
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1: NIH 3T3 whole cell lysate at 10 µg
Lane 2: CT26 whole cell lysate at 10 µg
Lane 3: CH27 whole cell lysate at 10 µg
Lane 4: TCMK-1 whole cell lysate at 10 µg
Lane 5: BW5147.3 whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 85 kDa
Observed band size: 90 kDa
Exposure time: 30s
Immunocytochemistry - Anti-CD44 antibody [BLR038F] (ab243894)
Immunocytochemical analysis of Formalin-Fixed Paraffin-Embedded 786-O cells labeling human CD44 with ab243894 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: DAB. Membrane staining on786-O cells is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.
Western blot - Anti-CD44 antibody [BLR038F] (ab243894)
All lanes: Western blot - Anti-CD44 antibody [BLR038F] (ab243894) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 10 µg
Lane 2: 786-O whole cell lysate at 10 µg
Lane 3: HEK293T whole cell lysate at 10 µg
Lane 4: A-549 whole cell lysate at 10 µg
Lane 5: KG-1 cells whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Developed using the ECL technique.
Predicted band size: 81 kDa
Observed band size: 90 kDa
Exposure time: 3s
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)
Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)
Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)
Flow cytometry overlay histogram of 4% formaldehyde fixed HeLa cells permeabilized with 90% methanol labeling human CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).
Flow Cytometry - Anti-CD44 antibody [BLR038F] (ab243894)
Flow cytometry overlay histogram of 4% formaldehyde fixed EL4 cells permeabilized with 90% methanol labeling mouse CD44 with ab243894 at 1 µl per 1 x 10^6 cells. (shaded) compared with a Isotype control (unshaded). Secondary antibody details: Goat Anti-Rabbit IgG H&L (DyLight® 650) (Goat Anti-Rabbit IgG H&L (DyLight® 650) ab96886).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)
Immunohistochemical analysis of Formalin-Fixed Paraffin-Embedded breast carcinoma labeling human CD44 with ab243894, followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: DAB. Membrane staining on breast carcinoma is observed. Counter stained with hematoxylin. Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [BLR038F] (ab243894)
Immunohistochemical (IHC-IF) analysis of Formalin-Fixed Paraffin-Embedded head and neck squamous cell carcinoma tissue labeling human CD44 (Orange) with ab243894 followed by a HRP-conjugated goat anti-rabbit IgG as secondary. Substrate: Opal™. Membrane staining on head and neck squamous cell carcinoma tissue is observed. Counter stained with DAPI (Blue). Epitope retrieval with citrate buffer pH 6.0 for 20 minutes is recommended.