Mouse Recombinant Monoclonal CD44 antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IP | Flow Cyt | WB | IHC-P | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.055 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.226 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.253 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
LHR, MDU2, MDU3, MIC4, CD44, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Mouse Recombinant Monoclonal CD44 antibody. Suitable for IP, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications.
IgG1
Mouse
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
C44Mab-5
Affinity purification Protein A
kappa
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
False colour image of Western blot: Anti-CD44 antibody [C44Mab-5] staining at 1.226 μg/ml, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab264539 was shown to bind specifically to CD44. A band was observed at 75-80 kDa in wild-type HeLa cell lysates with no signal observed at this size in CD44 knockout cell line Human CD44 knockout HeLa cell line ab262515 (knockout cell lysate Human CD44 knockout HeLa cell lysate ab263938). To generate this image, wild-type and CD44 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) at 1/20000 dilution.
All lanes: Western blot - Anti-CD44 antibody [C44Mab-5] (ab264539) at 1.226 µg/mL
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: CD44 knockout HeLa cell lysate at 20 µg
Lane 3: A549 cell lysate at 20 µg
Lane 4: LNCaP cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 75-80 kDa
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab264539 pre-coupled to prot.G-Sepharose beads. Samples were washed and processed for western blot with Anti-CD44 antibody [EPR18668] ab189524 at 1/2000. This data was kindly provided by the YCharOS Inc., an open science company with the mission of characterizing every commercially available antibody reagent. Abcam are working with YCharOS to support their mission of antibody characterisation using knock out cell lines.
All lanes: Immunoprecipitation - Anti-CD44 antibody [C44Mab-5] (ab264539)
Predicted band size: 81 kDa
Blocking/diluting buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab264539).
All lanes: Western blot - Anti-CD44 antibody [C44Mab-5] (ab264539) at 1.226 µg/mL
Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 81 kDa
Observed band size: 82 kDa
Exposure time: 70s
Immunoprecipitation of CD44 in HAP1 cells. Lysates were prepared and immunoprecipitation was performed using 1.0 μg of ab264539 pre-coupled to prot.G-Sepharose beads. Samples were washed and processed for western blot with Anti-CD44 antibody [EPR18668] ab189524 at 1/2000. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitate. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Immunoprecipitation - Anti-CD44 antibody [C44Mab-5] (ab264539)
Predicted band size: 81 kDa
Formalin-fixed, paraffin-embedded Human lung carcinoma tissue stained for CD44 using ab264539 at 0.253 μg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on Human lung carcinoma. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Flow cytometric analysis of MDA-MB-231 (Human breast adenocarcinoma epithelial cell) cell line labeling CD44 (Red) using ab264539 at 1.055 μg/mL followed by Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution. Mouse monoclonal IgG was used as the isotype control (Black). Cells without incubation with primary antibody and secondary antibody (Blue).
Gated on viable cells.
Formalin-fixed, paraffin-embedded Human skin tissue stained for CD44 using ab264539 at 0.253 μg/mL followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) in immunohistochemical analysis. Counterstained with Hematoxylin. Membranous staining on human skin. The section was incubated with ab264539 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Used PBS instead of the primary antibody, secondary antibody was a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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