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Rabbit Recombinant Monoclonal CD44 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 39 publications.


Images

Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647), expandable thumbnail
  • Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (AB216647), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PIPFlow CytWB
Human
Not recommended
Tested
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

4 products for Alternative Version

Target data

Function

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).

Alternative names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

Recommended products

Rabbit Recombinant Monoclonal CD44 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 39 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR1013Y
Purification technique
Affinity purification Protein A
Dissociation constant
3.76 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab216647 is the carrier-free version of Anti-CD44 antibody [EPR1013Y] ab51037.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    False colour image of Western blot: Anti-CD44 antibody [EPR1013Y] staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-CD44 antibody [EPR1013Y] ab51037 was shown to bind specifically to CD44. A band was observed at 75-80 kDa in wild-type HeLa cell lysates with no signal observed at this size in CD44 knockout cell line Human CD44 knockout HeLa cell line ab262515 (knockout cell lysate Human CD44 knockout HeLa cell lysate ab263938). To generate this image, wild-type and CD44 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-CD44 antibody [EPR1013Y] (Anti-CD44 antibody [EPR1013Y] ab51037) at 1/5000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CD44 knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human CD44 knockout HeLa cell line (Human CD44 knockout HeLa cell line ab262515)

    Lane 3: A549 cell lysate at 20 µg

    Lane 4: LNCaP cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 81 kDa

    Observed band size: 75-80 kDa

  • Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Western blot - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-CD44 antibody [EPR1013Y] ab51037).

    Lanes 1 - 4: Merged signal (red and green). Green - Anti-CD44 antibody [EPR1013Y] ab51037 observed at 80 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    Anti-CD44 antibody [EPR1013Y] ab51037 was shown to react with CD44 in wild-type HeLa cells in western blot. Loss of signal was observed when CD44 knockout sample was used. Wild-type HeLa and CD44 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-CD44 antibody [EPR1013Y] ab51037 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-CD44 antibody [EPR1013Y] (Anti-CD44 antibody [EPR1013Y] ab51037) at 1/5000 dilution

    Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: CD44 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Western blot - Human CD44 knockout HeLa cell line (Human CD44 knockout HeLa cell line ab262515)

    Lane 3: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 4: LNCaP (Human prostate cancer cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 81 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44 with Anti-CD44 antibody [EPR1013Y] ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD44 antibody [EPR1013Y] ab51037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling CD44 with Anti-CD44 antibody [EPR1013Y] ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD44 antibody [EPR1013Y] ab51037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue labeling CD44 with Anti-CD44 antibody [EPR1013Y] ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). The sample was counter stained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD44 antibody [EPR1013Y] ab51037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    Anti-CD44 antibody [EPR1013Y] ab51037 (1:100) showing positive staining in human Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD44 antibody [EPR1013Y] ab51037).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    Anti-CD44 antibody [EPR1013Y] ab51037 (1:100) showing positive staining in human Thyroid gland carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD44 antibody [EPR1013Y] ab51037).

  • OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    OI-RD Scanning - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    This IHC data was generated using the same anti-CD44 antibody clone, EPR1013Y, in a different buffer formulation (cat# Anti-CD44 antibody [EPR1013Y] ab51037).

    Anti-CD44 antibody [EPR1013Y] ab51037 (1:100) showing positive staining in human Breast carcinoma tissue.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR1013Y] - BSA and Azide free (ab216647)

    This IHC data was generated using the same anti-CD44 antibody clone, EPR1013Y, in a different buffer formulation (cat# Anti-CD44 antibody [EPR1013Y] ab51037).

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD44 with Anti-CD44 antibody [EPR1013Y] ab51037 at 1/100 dilution followed by goat anti-rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/500). The sample was counter stained with hematoxylin.

    Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

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