Anti-CD44 antibody [F10-44-2] (ab6124) is a mouse monoclonal antibody detecting CD44 in Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Over 70 publications
- Trusted since 2004
Images
![Flow Cytometry - Anti-CD44 antibody [F10-44-2] (AB6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--flow-cytometry-img121445.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD44 antibody [F10-44-2] (AB6124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (AB6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135896.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (AB6124)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (AB6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunocytochemistry-immunofluorescence-img184904.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (AB6124)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (AB6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26298.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (AB6124)")
Publications
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- Frontiers in cell and developmental biology 11:12589932023Combined 3D bioprinting and tissue-specific ECM system reveals the influence of brain matrix on stem cell differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMartina Zamponi,Peter A Mollica,Yara Khodour,Julie S Bjerring,Robert D Bruno,Patrick C SachsPubMed 37928905
- Macromolecular bioscience 23:e23001932023Dermatan Sulfate/Chitosan Nanoparticles Loaded with an Anti-Inflammatory Peptide Increase the Response of Human Colorectal Cancer Cells to 5-Fluorouracil.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgustín Blachman,Ariadna María Birocco,Sofía Curcio,Silvia Andrea Camperi,Paolo Di Gianvincenzo,Jésica Ayelén Rodriguez,Gabriela Romina Barredo-Vacchelli,Gloria Cenci,Alejandro Sosnik,Sergio Moya,Graciela Cristina CalabresePubMed 37469233
- Advances in rheumatology (London, England) 63:272023Effect of curcumin on the expression of NOD2 receptor and pro-inflammatory cytokines in fibroblast-like synoviocytes (FLSs) of rheumatoid arthritis (RA) patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFereshteh Akbari-Papkiadehi,Ali Akbar Saboor-Yaraghi,Elham Farhadi,Mohammad Naghi Tahmasebi,Arash Sharafat Vaziri,Leila Aghaghazvini,Marzieh Asgari,Shiva Poursani,Fatemeh Mansouri,Ahmadreza Jamshidi,Mahdi MahmoudiPubMed 37370181
- Frontiers in cell and developmental biology 11:11589362023Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianyu Cheng,Chang Liu,Qiuman Chen,Wenping Luo,Tong-Chuan He,Deqin YangPubMed 37283947
- Neuropathology and applied neurobiology 49:e129132023Increased number and domain of interlaminar astrocytes in layer I of the temporal cortex in epilepsy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Zhou,Zhen Fan,Yusheng Tong,Xing Xiao,Yongsheng Xie,Zengxin Qi,Liang ChenPubMed 37209022
- Frontiers in oncology 13:11476682023A recombinant adenovirus vector containing the receptor gene for the treatment of triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuhan A,Naoto Kunimura,Shoko Tominaga,Erika Hirata,Shunya Nishioka,Misato Uesugi,Rion Yamazaki,Hideto Ueki,Koichi Kitagawa,Masato Fujisawa,Toshiro ShirakawaPubMed 37064130
- Heliyon 9:e146552023The roles and molecular mechanisms of long non-coding RNA WT1-AS in the maintenance and development of gastric cancer stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobei Zhang,Meng Jin,Shiqi Liu,Mingde Zang,Lei Hu,Tao Du,Baogui ZhangPubMed 37025896
- Glia 71:1176-11962023Dysregulated cholesterol metabolism, aberrant excitability and altered cell cycle of astrocytes in fragile X syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaiyan Ren,Maria Burkovetskaya,Yoosun Jung,Lara Bergdolt,Steven Totusek,Veronica Martinez-Cerdeno,Kelly Stauch,Zeljka Korade,Anna DunaevskyPubMed 36594399
- Frontiers in oncology 12:10770242022Stem-cell therapy gastroscopy improves the outcome of esophageal anastomotic leakage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYannan Hu,Heng Chu,Xiang Xue,Yan Yan,Wenbang Chen,Xilong Lang,Hao ZhangPubMed 36605441
- Cancers 14:2022Immunohistochemical Markers of the Epithelial-to-Mesenchymal Transition (EMT) Are Related to Extensive Lymph Nodal Spread, Peritoneal Dissemination, and Poor Prognosis in the Microsatellite-Stable Diffuse Histotype of Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniele Marrelli,Luigi Marano,Maria Raffaella Ambrosio,Ludovico Carbone,Luigi Spagnoli,Roberto Petrioli,Alessandra Ongaro,Stefania Piccioni,Daniele Fusario,Franco RovielloPubMed 36551509
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | Flow Cyt | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg for 106 Cells | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
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Target data
Function
The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Recommended products
Anti-CD44 antibody [F10-44-2] (ab6124) is a mouse monoclonal antibody detecting CD44 in Flow Cytometry, IHC-P, ICC/IF. Suitable for Human.
- Over 70 publications
- Trusted since 2004
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- F10-44-2
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-CD44 antibody [F10-44-2] (ab6124) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.
Trusted by the scientific community
Anti-CD44 [F10-44-2] (ab6124) was first used in a scientific publication in 2004 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-CD44 [F10-44-2] (ab6124) has over 5 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [F10-44-2] also available for your convenience:
ab6124, FITC - FITC Anti-CD44 antibody [F10-44-2] ab30405, Carrier free - Anti-CD44 antibody [F10-44-2] - BSA and Azide free ab237970
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
![Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--flow-cytometry-img121445.jpg "Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)")
Flow Cytometry - Anti-CD44 antibody [F10-44-2] (ab6124)
Overlay histogram showing peripheral blood lymphocytes stained with ab6124 (red line). The cells were incubated with the antibody (ab6124, 0.5μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/200 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135896.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)")
Image from Le Bras GF et al., PLoS One. 2011;6(11):e27063. Epub 2011 Nov 1. Fig 3.; doi:10.1371/journal.pone.0027063; November 1, 2011, PLoS ONE 6(11): e27063.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
Immunohistochemical analysis of Human esophogeal keratinocytes, staining CD44 with ab6124.
Keratinocytes were cultured in vivo to form an epithelium for 15 days before fixing in formaldehyde and embedding in paraffin. Primary antibody was incubated overnight at 4°C and secondary antibody for 30 minutes at 37°C. Staining was detected using DAB.![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunocytochemistry-immunofluorescence-img184904.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [F10-44-2] (ab6124)
ab6124 staining CD44 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab6124 at 1µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-f10-44-2-ab6124--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img26298.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [F10-44-2] (ab6124)
IHC image of CD44 staining in human kidney carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6124, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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