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AB25340

Anti-CD44 antibody [KM201]

4

(2 Reviews)

|

(30 Publications)

Rat Monoclonal CD44 antibody. Suitable for Flow Cyt, IHC-FoFr and reacts with Mouse samples. Cited in 30 publications.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

1 Images
Flow Cytometry - Anti-CD44 antibody [KM201] (AB25340)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD44 antibody [KM201] (AB25340)

Flow Cytometry analysis of BALB/c mouse bone marrow cells labeling CD44 with ab25340 at 1 μg/106 cells dilution (purple). A Mouse Anti-Rat IgG1-FITC was used as the secondary antibody. Grey - Isotype Control, Rat IgG1-UNLB, followed by Mouse Anti-Rat IgG1-FITC.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KM201

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, IHC-FoFr

applications

Epitope

ab25340 reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "<p></p>" } } }
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Product details

ab25340 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and WhitlockWitte Cultures, prevent the earliest intrathymic precursors from homing to the thymus and costimulate the activation of freshly purified splenic CD4+ T cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 8.2 Constituents: 100% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.
See full target information CD44
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Publications (30)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:1015 PubMed37803211

2023

Dicalcin suppresses invasion and metastasis of mammalian ovarian cancer cells by regulating the ganglioside-Erk1/2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Naofumi Miwa,Mayu Hanaue,Kayo Aoba,Ryohei Saito,Ken Takamatsu

eLife 10: PubMed34110284

2021

Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Shan,Leike Li,Constance Lynn Atkins,Meng Wang,Yankai Wen,Jongmin Jeong,Nicolas F Moreno,Dechun Feng,Xun Gui,Ningyan Zhang,Chun Geun Lee,Jack A Elias,William M Lee,Bin Gao,Fong Wilson Lam,Zhiqiang An,Cynthia Ju

European journal of dentistry : PubMed33511601

2021

The Effect of Anadara granosa Shell's-Stichopus hermanni Scaffold on CD44 and IL-10 Expression to Decrease Osteoclasts in Socket Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Rima Parwati Sari,Syamsulina Revianti,Dwi Andriani,Widyasri Prananingrum,Retno Pudji Rahayu,Sri Agus Sudjarwo

Nature chemistry 12:929-938 PubMed32747755

2020

CD44 regulates epigenetic plasticity by mediating iron endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sebastian Müller,Fabien Sindikubwabo,Tatiana Cañeque,Anne Lafon,Antoine Versini,Bérangère Lombard,Damarys Loew,Ting-Di Wu,Christophe Ginestier,Emmanuelle Charafe-Jauffret,Adeline Durand,Céline Vallot,Sylvain Baulande,Nicolas Servant,Raphaël Rodriguez

Nature communications 11:2761 PubMed32487994

2020

Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Toda,Miriam Diaz-Varela,Joan Segui-Barber,Wanlapa Roobsoong,Barbara Baro,Susana Garcia-Silva,Alicia Galiano,Melisa Gualdrón-López,Anne C G Almeida,Marcelo A M Brito,Gisely Cardoso de Melo,Iris Aparici-Herraiz,Carlos Castro-Cavadía,Wuelton Marcelo Monteiro,Eva Borràs,Eduard Sabidó,Igor C Almeida,Jakub Chojnacki,Javier Martinez-Picado,Maria Calvo,Pilar Armengol,Jaime Carmona-Fonseca,Maria Fernanda Yasnot,Ricardo Lauzurica,Antonio Marcilla,Hector Peinado,Mary R Galinski,Marcus V G Lacerda,Jetsumon Sattabongkot,Carmen Fernandez-Becerra,Hernando A Del Portillo

Stem cell research & therapy 10:366 PubMed31791401

2019

The role of pulmonary mesenchymal cells in airway epithelium regeneration during injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Suyun Fang,Suhong Zhang,Haiting Dai,Xiaoxiang Hu,Changgong Li,Yiming Xing

Scientific reports 8:16058 PubMed30375477

2018

Molecular weight of surface immobilized hyaluronic acid influences CD44-mediated binding of gastric cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Amorim,Diana Soares da Costa,Daniela Freitas,Celso A Reis,Rui L Reis,Iva Pashkuleva,Ricardo A Pires

Stem cells (Dayton, Ohio) 36:1525-1534 PubMed29873154

2018

ESRP1-Induced CD44 v3 Is Important for Controlling Pluripotency in Human Pluripotent Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Young-Dae Kim,Han-Seop Kim,Jungwoon Lee,Jung-Kyun Choi,Enna Han,Ji E Jeong,Yee S Cho

Neuron 99:702-719.e6 PubMed30078576

2018

Extracellular Matrix Components HAPLN1, Lumican, and Collagen I Cause Hyaluronic Acid-Dependent Folding of the Developing Human Neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine R Long,Ben Newland,Marta Florio,Nereo Kalebic,Barbara Langen,Anna Kolterer,Pauline Wimberger,Wieland B Huttner

Immunity 49:326-341.e7 PubMed30054204

2018

Hyaluronan Receptor LYVE-1-Expressing Macrophages Maintain Arterial Tone through Hyaluronan-Mediated Regulation of Smooth Muscle Cell Collagen.

Applications

Unspecified application

Species

Unspecified reactive species

Hwee Ying Lim,Sheau Yng Lim,Chek Kun Tan,Chung Hwee Thiam,Chi Ching Goh,Daniel Carbajo,Samantha Hui Shang Chew,Peter See,Svetoslav Chakarov,Xiao Nong Wang,Li Hui Lim,Louise A Johnson,Josephine Lum,Chui Yee Fong,Ariff Bongso,Arijit Biswas,Chern Goh,Maximilien Evrard,Kim Pin Yeo,Ranu Basu,Jun Kit Wang,Yingrou Tan,Rohit Jain,Shweta Tikoo,Cleo Choong,Wolfgang Weninger,Michael Poidinger,Richard E Stanley,Matthew Collin,Nguan Soon Tan,Lai Guan Ng,David G Jackson,Florent Ginhoux,Véronique Angeli
View all publications
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Product promise

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