Mouse Monoclonal CD44 antibody. Extracellular domain. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD44.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (AB253275), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-m007-extracellular-domain-ab253275--immunocytochemistry-immunofluorescence-img99722.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (AB253275)")
![Western blot - Anti-CD44 antibody [M007] - Extracellular domain (AB253275), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-m007-extracellular-domain-ab253275--western-blot-img99464.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD44 antibody [M007] - Extracellular domain (AB253275)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human CD44. The exact immunogen used to generate this antibody is proprietary information. Database link P16070
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Target data
Function
Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
Alternative names
CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I
Recommended products
Mouse Monoclonal CD44 antibody. Extracellular domain. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD44.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human CD44. The exact immunogen used to generate this antibody is proprietary information. Database link P16070
- Clone number
- M007
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (ab253275), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-m007-extracellular-domain-ab253275--immunocytochemistry-immunofluorescence-img99722.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)")
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)
Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells labeling CD44 using ab253275 at 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.
![Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275), expandable thumbnail](https://content.abcam.com/products/images/cd44-antibody-m007-extracellular-domain-ab253275--western-blot-img99464.jpg "Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)")
Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275)
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hour at room temperature.
All lanes: Western blot - Anti-CD44 antibody [M007] - Extracellular domain (ab253275) at 1/1000 dilution
Lane 1: A549 (human lung carcinoma cell line) cell lysate, native conditions
Lane 2: A549 (human lung carcinoma cell line) cell lysate, denatured conditions
Lane 3: MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate, native conditions
Lane 4: MDA-MB-231 (human breast adenocarcinoma cell line) cell lysate, denatured conditions
Predicted band size: 81 kDa
Downloads
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- Visit the general protocols
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