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AB253276

Anti-CD44 antibody [M010] - Extracellular domain

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(1 Publication)

Mouse Monoclonal CD44 antibody. Extracellular domain. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD44.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M010] - Extracellular domain (AB253276)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M010] - Extracellular domain (AB253276)

Paraformaldehyde-fixed A431 (Human epidermoid carcinoma cell line) cells labeling CD44 using ab253276 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

Western blot - Anti-CD44 antibody [M010] - Extracellular domain (AB253276)
  • WB

Supplier Data

Western blot - Anti-CD44 antibody [M010] - Extracellular domain (AB253276)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

All lanes:

Western blot - Anti-CD44 antibody [M010] - Extracellular domain (ab253276) at 1/1000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) whole cell lysate, native conditions

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate, denatured conditions

Lane 3:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, native conditions

Lane 4:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, denatured conditions

Predicted band size: 81 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M010

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CD44. The exact immunogen used to generate this antibody is proprietary information.

P16070

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.
See full target information CD44
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 15:959224 PubMed36245919

2022

Repeated inhibition of sigma-1 receptor suppresses GABA receptor expression and long-term depression in the nucleus accumbens leading to depressive-like behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Qin,Weixing Xu,Kunpeng Li,Qi Luo,Xi Chen,Yue Wang,Lei Chen,Sha Sha
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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