Anti-CD44 antibody [M024] - Extracellular domain

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M024] - Extracellular domain (AB253277)

Paraformaldehyde-fixed MeWo (Human malignant melanoma cell line) cells labeling CD44 using ab253277 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight^® 594 secondary.

• ELISA

Supplier Data

ELISA - Anti-CD44 antibody [M024] - Extracellular domain (AB253277)

Representative Standard Curve using mouse monoclonal anti-CD44 for ELISA capture of human recombinant CD44 extracellular region with His-tag. Capture was detected by using an anti-His-tag antibody followed by appropriate secondary antibody conjugated to HRP.

• WB

Supplier Data

Western blot - Anti-CD44 antibody [M024] - Extracellular domain (AB253277)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

All lanes:

Western blot - Anti-CD44 antibody [M024] - Extracellular domain (ab253277) at 1/2000 dilution

Lane 1:

A549 (Human lung carcinoma cell line) cell lysate

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma cell line) cell lysate

Lane 3:

MeWo (Human malignant melanoma cell line) cell lysate

Lane 4:

Human breast tissue lysate

Lane 5:

Human lung tissue lysate

Lane 6:

Human skin tissue lysate

Lane 7:

Human brain tissue lysate

Predicted band size: 81 kDa

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