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AB253279

Anti-CD44 antibody [M591]

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Mouse Monoclonal CD44 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CD44.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M591] (AB253279)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD44 antibody [M591] (AB253279)

Paraformaldehyde-fixed MCF7 (Human breast adenocarcinoma cell line) cells labeling CD44 using ab253279 at a 1/100 dilution in ICC/IF. Goat anti-mouse DyLight® 594 secondary.

Western blot - Anti-CD44 antibody [M591] (AB253279)
  • WB

Supplier Data

Western blot - Anti-CD44 antibody [M591] (AB253279)

Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween-20 for 1 hr at room temperature.

All lanes:

Western blot - Anti-CD44 antibody [M591] (ab253279) at 1/1000 dilution

Lane 1:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, denatured conditions

Lane 2:

MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate, native conditions

Predicted band size: 81 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

M591

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CD44. The exact immunogen used to generate this antibody is proprietary information.

P16070

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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AB287168

Human CD44 ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The protein expressed by gene CD44 functions as a cell-surface receptor involved in cell-cell interactions, cell adhesion, and migration, allowing cells to detect and react to changes in their tissue microenvironment. It is implicated in numerous cellular processes, including the activation, recirculation, and homing of T-lymphocytes, hematopoiesis, inflammation, and the response to bacterial infection. Through its ectodomain, CD44 interacts with extracellular matrix components like hyaluronan, collagen, growth factors, cytokines, and proteases, serving as a platform for signal transduction by forming protein complexes with receptor kinases and membrane proteases via its cytoplasmic domain. Effectors such as PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases, and phospholipase C coordinate signaling pathways that promote calcium mobilization and actin-mediated cytoskeleton reorganization crucial for cell migration and adhesion. This supplementary information is collated from multiple sources and compiled automatically.
See full target information CD44
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com