AB255931

Anti-CD44v6 antibody [2F10] - BSA and Azide free

Recombinant
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Mouse Recombinant Monoclonal CD44 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.

View Alternative Names

CD44, LHR, MDU2, MDU3, MIC4, CD44 antigen, CDw44, Epican, Extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Heparan sulfate proteoglycan, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, Phagocytic glycoprotein I, ECMR-III, PGP-1, PGP-I

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Immunohistochemical analysis of paraffin-embedded human breast tissue labeling CD44v6 with ab254174 at 3.73μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast tissue is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This image was produced using the same antibody clone but in a different formulation ab254174, PBS, sodium azide, glycerol and BSA.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling CD44v6 with ab254174 at 3.73μg/ml, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human breast carcinoma tissue is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This image was produced using the same antibody clone but in a different formulation ab254174, PBS, sodium azide, glycerol and BSA.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling CD44v6 with ab254174 at 3.73μg/ml, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Membranous staining on human skin tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This image was produced using the same antibody clone but in a different formulation ab254174, PBS, sodium azide, glycerol and BSA.

Unconjugated

Anti-CD44v6 antibody [2F10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2F10

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3.73 µg/mL", "IHCP-species-notes": "" } } }

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Product details

ab255931 is the carrier-free version of ab254174.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed : 16541107, PubMed : 19703720, PubMed : 22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed : 7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed : 18757307, PubMed : 23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed : 15123640). Upon interaction with LGALS9 ligand, activates downstream signaling components including LCK, ERK and MAPK to promotes NK cell activation (PubMed : 37006235).. (Microbial infection) Promotes foot-and-mouth disease virus internalization via macropinocytosis.

See full target information CD44

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD44v6 antibody [2F10] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44v6 antibody [2F10] - BSA and Azide free (AB255931)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Product promise