AB154885

Anti-CD45 antibody

Name: Anti-CD45 antibody (ab154885) | Abcam
Brand: Abcam Limited
SKU: AB154885

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(5 Publications)

Rabbit Polyclonal CD45 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTPRC aa 900-1050.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (AB154885)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody (AB154885)

Immunofluorescence analysis of THP-1 cells labeling CD45 with ab154885 at 1/500 dilution. Hoechst 33342 (blue) staining is used (right panel).

Flow Cytometry - Anti-CD45 antibody (AB154885)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45 antibody (AB154885)

Flow cytometric analysis of THP-1 cells labelling CD45 protein with ab154885 at 1/50 dilution (Red) compared with unlabelled sample (Black).

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45 antibody (AB154885)

Flow cytometric analysis of Mouse splenocytes cell fixed in 4% paraformaldehyde at 4°C for 15 min labelling CD45 protein with ab154885 at 1/50 dilution (Red) compared with unlabelled sample (Black).
Acquisition of 20,000 events were collected for FACS analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PTPRC aa 900-1050. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Product details

This antibody provides variable results in WB.

Keep as concentrated solution.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37500179

2023

Inhibition of protein arginine deiminase 4 prevents inflammation-mediated heart failure in arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas A Heger,Nicolas Schommer,Shoichi Fukui,Stijn Van Bruggen,Casey E Sheehy,Long Chu,Sridharan Rajagopal,Dhanalakshmi Sivanandhan,Bruce Ewenstein,Denisa D Wagner

Stem cell research & therapy 14:89 PubMed37061739

2023

Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Sahoo,Mukesh Damala,Jilu Jaffet,Deeksha Prasad,Sayan Basu,Vivek Singh

Stem cells international 2022:4775445 PubMed36110889

2022

Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Sun,Jingfei Fu,Feiran Lin,Shengnan Li,Juan Du,Yi Liu,Yuxing Bai

Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology 41:123-132 PubMed31568132

2019

Toll-like Receptor 4 Signaling and Downstream Neutrophilic Inflammation Mediate Endotoxemia-Enhanced Blood-Labyrinth Barrier Trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary D Urdang,Jessica L Bills,David Y Cahana,Leslie L Muldoon,Edward A Neuwelt

PloS one 10:e0116270 PubMed25643152

2015

Alpha-enolase is upregulated on the cell surface and responds to plasminogen activation in mice expressing a ∆133p53α mimic.

Applications

Species

Mouse

Sonal Sawhney,Kylie Hood,Alisha Shaw,Antony W Braithwaite,Richard Stubbs,Noelyn A Hung,Janice A Royds,Tania L Slatter

View all publications

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