AB243869

Anti-CD45 antibody [BL-178-12C7]

Recombinant
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(3 Publications)

Rabbit Recombinant Monoclonal CD45 antibody. Suitable for IHC-P, ICC, IP, Flow Cyt, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTPRC aa 1250 to C-terminus.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (AB243869)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Formalin-fixed, paraffin-embedded human small intestine tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Unknown

Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Formaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD45 using ab243869 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Formalin-fixed, paraffin-embedded human appendix tissue stained for CD45 using ab243869 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Flow cytometric analysis of 4% formaldehyde and permeabilized with 90% methanol Jurkat (Human T cell leukemia cell line from peripheral blood) cells labelling CD45 with ab243869 at 1μl/10⁶ cells (Red shaded) compared with a Isotype control (unshaded). DyLight^® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (AB243869)

CD45 was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243869 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243869 at 1/1000 dilution. Lane 1 : ab243869 IP in Jurkat cell lysate.

Lane 2 : Contol IgG in Jurkat cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Predicted band size: 147 kDa

false

Unknown

Western blot - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Western blot analysis using ab243869 at 1/1000 dilution.

Lane 1 : Jurkat whole cell lysate (50 µg).

Lane 2 : MOLT-4 whole cell lysate (50 µg).

Lane3 : K562 whole cell lysate (50 µg).

Lane 4 : SR whole cell lysate (50 µg).

Lane 5 : HeLa whole cell lysate (50 µg).

Lane 6 : HEK-293T whole cell lysate (50 µg).

A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection : chemiluminescence with an exposure time of 3 seconds

All lanes:

Western blot - Anti-CD45 antibody [BL-178-12C7] (ab243869)

Predicted band size: 147 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BL-178-12C7

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, IP, Flow Cyt, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PTPRC aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 20μl/mg lysate.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form

Liquid

Purification notes

Recombinant antibody was purified from cell culture supernatant.

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 20:8765-8781 PubMed40635718

2025

Boosting Hydroxyl Radical Generation with Nitrogen Vacancy-Modified Carbon Nitride for Triggering Dual Damage of Cancer Nucleus DNA-Mitochondria against Hypoxic Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Meixian Liu,Shuyun Xue,Na Li,Jinjia Hong,Jiawen Gu,Xiaoyan Ren,Huifei Li,Jie Xu,Chengxi Zhang,Tianqi Liang,Bin Zhao,Xing Wang

Journal of orthopaedic surgery and research 20:336 PubMed40176111

2025

USP15-modified ADMSCs-Exo alleviates chondrocyte damage and effectively relieved osteoarthritis by inducing M2 polarization of macrophages through deubiquitinating FOXC1.

Applications

Unspecified application

Species

Unspecified reactive species

Qibin Liang,Qinghe Ding,Liang Zhao,Jingchao Tan,Wei Niu

BMC oral health 24:407 PubMed38556862

2024

KAT2A-mediated succinylation modification of notch1 promotes the proliferation and differentiation of dental pulp stem cells by activating notch pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Longwei Ye,Zeqin Yu,Lin He,Jie Yuan,Xiaodan Zhang,Lei Li,Xin Huang,Yanyan Ma,Lei Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD45 antibody [BL-178-12C7]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Immunocytochemistry - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Flow Cytometry - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Immunoprecipitation - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Western blot - Anti-CD45 antibody [BL-178-12C7] (AB243869)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

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Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise