JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10559

Anti-CD45 antibody - Hematopoietic Stem Cell Marker

5

(7 Reviews)

|

(45 Publications)

Rabbit Polyclonal CD45 antibody. Hematopoietic Stem Cell marker. Suitable for IHC-P, WB and reacts with Human samples. Cited in 45 publications.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (AB10559)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (AB10559)

IHC image of ab10559 staining in human Hodgkins lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10559, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (AB10559)
  • WB

Unknown

Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (AB10559)

ab10559 rabbit polyclonal to CD45 (1/500) with secondary goat anti-rabbit IgG ab6721 (1/5000).

Expected molecular weight : 147 kDa. WB exposure time : 1min 30sec

Lanes 1 to 10 : 20μg of cell lysate per lane
Lanes 6 to 10 : ab17550 CD45 blocking peptide used at 1μg/ml

Lane 1 : HeLa Nuclear Extract (ab10559)
Lane 2 : HeLa Whole Cell Lysate (ab10559)
Lane 3 : A431 Whole Cell Lysate (ab10559)
Lane 4 : Jurkat Whole Cell Lysate (ab10559)
Lane 5 : HEK293 Whole Cell Lysate (ab10559)
Lane 6 : HeLa Nuclear Extract (ab10559+ ab17550)
Lane 7 : HeLa Whole Cell Lysate (ab10559+ ab17550)
Lane 8 : A431 Whole Cell Lysate (ab10559+ ab17550)
Lane 9 : Jurkat Whole Cell Lysate (ab10559+ ab17550)
Lane 10 : HEK293 Whole Cell Lysate (ab10559+ ab17550)

A strong band, slightly higher was seen in Jurkat cell lysate. The band was blocked using the immunising peptide (ab17550). It is likely that the band is CD45.

All lanes:

Western blot - Anti-CD45 antibody - Hematopoietic Stem Cell Marker (ab10559)

Predicted band size: 147 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
style
left-column

Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Acta neuropathologica 149:39 PubMed40299057

2025

Spatial mapping of the AA-PGE-EP axis in multiple sclerosis lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Cathrin E Hansen,Julia Konings,Gabor Toth,Serhii Chornyi,Manon Karsten,Bert van Het Hof,Susanne M A van der Pol,Stephanie D Beekhuis-Hoekstra,Nine Kok,Wing Ka Fung,Naomi S Dijksman,Wia Baron,Maarten E Witte,Ingela Lanekoff,Helga E de Vries,Gijs Kooij

NAR genomics and bioinformatics 6:lqae098 PubMed40162103

2025

Evaluating cell type deconvolution in FFPE breast tissue: application to benign breast disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanhang Liu,Robert A Vierkant,Aditya Bhagwate,William A Jons,Melody L Stallings-Mann,Bryan M McCauley,Jodi M Carter,Melissa T Stephens,Michael E Pfrender,Laurie E Littlepage,Derek C Radisky,Julie M Cunningham,Amy C Degnim,Stacey J Winham,Chen Wang

iScience 27:109928 PubMed38812546

2024

Single-cell transcriptomic-informed deconvolution of bulk data identifies immune checkpoint blockade resistance in urothelial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Sudeh Izadmehr,John P Sfakianos,Michelle Tran,Kristin G Beaumont,Rachel Brody,Carlos Cordon-Cardo,Amir Horowitz,Robert Sebra,William K Oh,Nina Bhardwaj,Matthew D Galsky,Jun Zhu

In vivo (Athens, Greece) 38:710-718 PubMed38418148

2024

External Volume Expansion: Timing and Effects on the Rate of Fat Graft Retention in BALB/c Nude Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yong June Chang,Yun Hyun Kim,Joon Seok Lee,Jeong Yeop Ryu,Kang Young Choi,Ho Yun Chung,Byung Chae Cho,Jung Dug Yang

The European respiratory journal 61: PubMed37105573

2023

Nicotine promotes e-cigarette vapour-induced lung inflammation and structural alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Elsa T Roxlau,Oleg Pak,Stefan Hadzic,Claudia F Garcia-Castro,Marija Gredic,Cheng-Yu Wu,Julia Schäffer,Balachandar Selvakumar,Alexandra Pichl,David Spiegelberg,Janik Deutscher,Mariola Bednorz,Katharina Schäfer,Simone Kraut,Djuro Kosanovic,Esraa M Zeidan,Baktybek Kojonazarov,Susanne Herold,Ievgen Strielkov,Andreas Guenther,Jochen Wilhelm,Mohamed M A Khalifa,Ashraf Taye,Ralf P Brandes,Matthias Hecker,Friedrich Grimminger,Hossein A Ghofrani,Ralph T Schermuly,Werner Seeger,Natascha Sommer,Norbert Weissmann

Journal of cell communication and signaling 17:1055-1065 PubMed37191838

2023

Optimized multiplex immunofluorescence for the characterization of tumor immune microenvironment in neoplastic paraffin-preserved tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Fernanda Marconi Roversi,Maura Lima Pereira Bueno,Juliete Aparecida Francisco da Silva,Guilherme Rossi Assis-Mendonça,Sara Teresinha Olalla Saad

Cornea 41:470-477 PubMed35244627

2022

Comparison of the Effects of Temperature and Dehydration Mode on Glycerin-Based Approaches to SMILE-Derived Lenticule Preservation.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xia,Jing Zhao,Dan Fu,Bing Qin,Zhi Chen,Yu Zhao,Yang Shen,Jiayun Hou,Xingtao Zhou

Molecular cell 82:833-851.e11 PubMed35180428

2022

HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Huacheng Luo,Ganqian Zhu,Melanie A Eshelman,Tsz Kan Fung,Qian Lai,Fei Wang,Bernd B Zeisig,Julia Lesperance,Xiaoyan Ma,Shi Chen,Nicholas Cesari,Christopher Cogle,Baoan Chen,Bing Xu,Feng-Chun Yang,Chi Wai Eric So,Yi Qiu,Mingjiang Xu,Suming Huang

Cells 11: PubMed35159330

2022

Nuclear Localization Sequence of FGF1 Is Not Required for Its Intracellular Anti-Apoptotic Activity in Differentiated Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Agata Lampart,Katarzyna Dominika Sluzalska,Aleksandra Czyrek,Aleksandra Szerszen,Jacek Otlewski,Antoni Wiedlocha,Malgorzata Zakrzewska

Oncology reports 47: PubMed34958112

2021

Systematic characterization of the tumor microenvironment in Chinese patients with hepatocellular carcinoma highlights intratumoral B cells as a potential immunotherapy target.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Feng,Liguo Liu,Jing Li,Jia Huang,Jenny H Xie,Laurence Menard,Yanfen Shi,Xiaohong Zhao,Shan Xie,Wenjuan Zang,Haidong Tan,Zhiying Yang,Ling Ni
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB40763

Anti-CD45 antibody [EP322Y]

20ul selling size|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] (AB40763)

  • 5
  • 14
Primary Antibodies

AB200315

Alexa Fluor® 488 Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-CD45 antibody [EP322Y] (AB200315)

  • 0
  • 0
Primary Antibodies

AB221245

APC Anti-CD45 antibody [EP322Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - APC Anti-CD45 antibody [EP322Y] (AB221245)

  • 0
  • 0
Primary Antibodies

AB208022

Anti-CD45 antibody [EPR20033]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] (AB208022)

  • 5
  • 2
Primary Antibodies

AB229292

Anti-CD45 antibody [EPR20033] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [EPR20033] - BSA and Azide free (AB229292)

  • 5
  • 2
Primary Antibodies

AB214437

Anti-CD45 antibody [EP322Y] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-CD45 antibody [EP322Y] - BSA and Azide free (AB214437)

  • 5
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 79
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 19
Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

  • 4
  • 48

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com