Anti-CD45 antibody [I3/2.3]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [I3/2.3] (AB25386)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse splenocytes cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing cytoplasmic staining in mouse splenocytes (shown in green). The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD45 antibody [I3/2.3] (AB25386)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast, Left) / WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD45 with ab25386 at 1/500 dilution (0.1 µg) / Right compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and Cell without incubation with primary antibody and secondary antibody / Blue.

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) was used as a secondary antibody at a 1/5000 dilution.

Negative control : Neuro-2a.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD45 antibody [I3/2.3] (AB25386)

Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Mouse PBMC cells labelling CD45 with ab25386 at 1/500 dilution (0.1 µg) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control

Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor^® 488, ab150161) was used as a secondary antibody at a 1/5000 dilution.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [I3/2.3] (AB25386)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in WEHI-231 cell line (shown in green), no staining was observed in Neuro-2a cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : Neuro-2a.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD45 antibody [I3/2.3] (AB25386)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CD45 with ab25386 at 1/100 dilution, followed by ab150161 Goat F(ab')2 Anti-Rat IgG Fc (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution(Green).

Confocal image showing membranous staining in J774A.1 cell line (shown in green), no staining was observed in C2C12 cell line. The counterstain was observed in red. Nuclear DNA was labeled with DAPI (shown in blue).
Negative control : C2C12.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab206369 Anti-beta Tubulin rabbit monoclonal antibody (Alexa Fluor^® 594) was used to counter stain Tubulin at a 1/200 dilution (Red).