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AB23910

Anti-CD45 antibody [IBL-3/16]

4

(1 Review)

|

(27 Publications)

Anti-CD45 antibody [IBL-3/16] (ab23910) is a rat monoclonal antibody detecting CD45 in Flow Cytometry, IP, IHC-Fr, ICC/IF. Suitable for Mouse.

- Over 20 publications
- Trusted since 2005

View Alternative Names

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

IBL-3/16

Isotype

IgG1

Carrier free

No

Reacts with

Mouse

Applications

IHC-Fr, ICC/IF, Flow Cyt, IP

applications

Immunogen

Cell preparation containing Ptprc protein. The exact immunogen used to generate this antibody is proprietary information.

P06800

Reactivity data

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Product details

What is this antibody validated in?
Anti-CD45 antibody [IBL-3/16] (ab23910) is a rat monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-CD45 [IBL-3/16] (ab23910) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

Properties and storage information

Form
Liquid
Purification notes
Ig fraction prepared by ammonium sulphate precipitation from tissue culture supernatant.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.
See full target information Ptprc

Publications (27)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0328001 PubMed40844997

2025

Ischemic duration determines extent of cardiac remodeling, and both early and delayed reperfusion prevent fatal cardiac rupture: Model comparison.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhao,Amanguli Ruze,Guo-Li Du,Min-Tao Gai,Jing Tang,Xiao-Ming Gao

Stem cell research & therapy 16:218 PubMed40312745

2025

Puerarin relives inflammation, bone destruction and facilitates osteogenic differentiation in periodontitis by enhancing mitochondrial autophagy via activating mitochondrial Mitofusin 2.

Applications

Unspecified application

Species

Unspecified reactive species

Yulan Xiang,Zelu Li,Xin He,Xiaoyang Chu,Chunyan Gao,Jiahao Guo,Yingyi Luan,Kai Yang,Dongliang Zhang

Journal of extracellular vesicles 14:e70038 PubMed39868462

2025

Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Busatto,Tzu-Hsi Song,Hyung Joon Kim,Caleb Hallinan,Michael N Lombardo,Anat O Stemmer-Rachamimov,Kwonmoo Lee,Marsha A Moses

Respiratory research 25:6 PubMed38178133

2024

CD147 induces asthmatic airway remodeling and activation of circulating fibrocytes in a mouse model of asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Li,Tao Cheng,Yaning Guo,Rong Gao,Xuankun Ma,Xuecong Mao,Xinpeng Han

Nature 623:792-802 PubMed37968392

2023

CD201 fascia progenitors choreograph injury repair.

Applications

Unspecified application

Species

Unspecified reactive species

Donovan Correa-Gallegos,Haifeng Ye,Bikram Dasgupta,Aydan Sardogan,Safwen Kadri,Ravinder Kandi,Ruoxuan Dai,Yue Lin,Robert Kopplin,Disha Shantaram Shenai,Juliane Wannemacher,Ryo Ichijo,Dongsheng Jiang,Maximilian Strunz,Meshal Ansari,Illias Angelidis,Herbert B Schiller,Thomas Volz,Hans-Günther Machens,Yuval Rinkevich

Advanced materials (Deerfield Beach, Fla.) 35:e2302801 PubMed37589156

2023

Cellular Membrane-Engineered Nanovesicles as a Three-Stage Booster to Target the Lesion Core.

Applications

Unspecified application

Species

Unspecified reactive species

Fanyi Huang,Yawei Du,Yanjia Chen,Zeping Qiu,Zhiyan Wang,Yingze Fan,Yunjing Shi,Zhuojin Li,Ke Yang,Wenguo Cui,Wei Jin

Frontiers in cellular neuroscience 17:1192464 PubMed37377777

2023

Delineating effects of angiopoietin-2 inhibition on vascular permeability and inflammation in models of retinal neovascularization and ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Jérémie Canonica,Richard Foxton,Marina Garcia Garrido,Cheng-Mao Lin,Sabine Uhles,Sumathi Shanmugam,David A Antonetti,Steven F Abcouwer,Peter D Westenskow

BMC veterinary research 19:64 PubMed36997964

2023

Mesenchymal stromal cells modulate infection and inflammation in the uterus and mammary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Iftach Schouten,Andrés Bernys-Karolys,Peleg Schneider,Tal Dror,Lior Ofer,Chen Shimoni,Einat Nissim-Eliraz,Nahum Y Shpigel,Sharon Schlesinger

Nature metabolism 4:995-1006 PubMed35902645

2022

Systemic induction of senescence in young mice after single heterochronic blood exchange.

Applications

Unspecified application

Species

Unspecified reactive species

Ok Hee Jeon,Melod Mehdipour,Tae-Hwan Gil,Minha Kang,Nicholas W Aguirre,Zachery R Robinson,Cameron Kato,Jessy Etienne,Hyo Gyeong Lee,Fatouma Alimirah,Vighnesh Walavalkar,Pierre-Yves Desprez,Michael J Conboy,Judith Campisi,Irina M Conboy

Frontiers in immunology 13:853769 PubMed35572508

2022

Microvesicle-Mediated Communication Within the Alveolar Space: Mechanisms of Uptake by Epithelial Cells and Alveolar Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sanooj Soni,Kieran P O'Dea,Eiko Abe,Maryam Khamdan,Sneh V Shah,Padmini Sarathchandra,Michael R Wilson,Masao Takata
View all publications

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