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AB33923

Anti-CD45 antibody [MRC OX-1]

4

(3 Reviews)

|

(15 Publications)

Mouse Monoclonal CD45 antibody. Suitable for IHC-Fr and reacts with Rat samples. Cited in 15 publications.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (AB33923)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD45 antibody [MRC OX-1] (AB33923)

IHC image of CD45 staining in a section of frozen normal rat spleen*.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.

Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33923 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33923.

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from Charles River.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Rat

Applications

IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 19:20240973 PubMed38919547

2024

Lycopene inhibits pyroptosis of endothelial progenitor cells induced by ox-LDL through the AMPK/mTOR/NLRP3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chujun Tan,Junqiu Chen,Tengcan Tu,Lifang Chen,Jun Zou

Bioactive materials 20:179-193 PubMed35663336

2022

A bioactive material with dual integrin-targeting ligands regulates specific endogenous cell adhesion and promotes vascularized bone regeneration in adult and fetal bone defects.

Applications

Unspecified application

Species

Unspecified reactive species

Dake Hao,Ruiwu Liu,Tomas Gonzalez Fernandez,Christopher Pivetti,Jordan Elizabeth Jackson,Edwin Samuel Kulubya,Hong-Jiang Jiang,Hai-Yang Ju,Wen-Liang Liu,Alyssa Panitch,Kit S Lam,J Kent Leach,Diana L Farmer,Aijun Wang

The FEBS journal 289:5279-5304 PubMed35285180

2022

HPS protects the liver against steatosis, cell death, inflammation, and fibrosis in mice with steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Xian Liu,Hui Chen,Pengjun Wang,Songhui Yao,Bin Zhou,Ronghua Yin,Changyan Li,Chutse Wu,Xiaoming Yang,Miao Yu

Stem cells (Dayton, Ohio) 39:1751-1765 PubMed34418223

2021

Spatiotemporal extracellular matrix modeling for in situ cell niche studies.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Olesen,Sergey Rodin,Wing Cheung Mak,Ulrika Felldin,Cecilia Österholm,Andreas Tilevik,Karl-Henrik Grinnemo

American journal of translational research 13:952-966 PubMed33841632

2021

Co-modification of calcium phosphate cement to achieve rapid bone regeneration in osteoporotic femoral condyle defect with lithium and aspirin.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Wan-Shu Zhou,Rou-Tian Zhang,Yang Li,Hong-Guang Xu,Shan Wei,Zheng-Yu Wang,Min Yang

mSphere 6: PubMed33692197

2021

Pathogenesis Study of Enterovirus 71 Using a Novel Human SCARB2 Knock-In Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yuefei Jin,Tiantian Sun,Guangyuan Zhou,Dong Li,Shuaiyin Chen,Weiguo Zhang,Xueyuan Li,Rongguang Zhang,Haiyan Yang,Guangcai Duan

Journal of orthopaedic translation 27:67-76 PubMed33437639

2021

Parathyroid hormone (1-34) can reverse the negative effect of valproic acid on the osseointegration of titanium rods in ovariectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhou-Shan Tao,Wan-Shu Zhou,Hong-Guang Xu,Min Yang

Experimental and therapeutic medicine 20:19 PubMed32934684

2020

Combined culture experiment of mouse bone marrow mesenchymal stem cells and bioceramic scaffolds.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Huang,Zhenhao Chen,Guanglei Zhao,Jingsheng Shi,Gangyong Huang,Feiyan Chen,Yibing Wei,Jun Xia,Jie Chen,Siqun Wang

Journal of cellular biochemistry 120:5570-5582 PubMed30417423

2018

IGF-1 and BMP-7 synergistically stimulate articular cartilage repairing in the rabbit knees by improving chondrogenic differentiation of bone-marrow mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Bo Peng,Yuan Zhang,Yue-Qiu Wang,Qi He,Qian Yu

European journal of histochemistry : EJH 61:2790 PubMed28735520

2017

Gemst: a taylor-made combination that reverts neuroanatomical changes in stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Arturo Mangas,Javier Yajeya,Noelia González,Isabel Ruiz,Marianny Pernìa,Michel Geffard,Rafael Coveñas
View all publications
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