AB781

Anti-CD45 antibody [PD7/26 + 2B11]

Name: Anti-CD45 antibody [PD7/26 + 2B11] (ab781) | Abcam
Brand: Abcam Limited
SKU: AB781
Price: 400 USD
Availability: InStock

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(11 Publications)

Mouse Multiclonal CD45 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human PTPRC.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (AB781)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45 antibody [PD7/26 + 2B11] (AB781)

Formalin fixed paraffin embedded human tonsil tissue, staining CD45 with ab781 in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Multiclonal

Clone number

PD7/26 + 2B11

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PTPRC. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/40 - 1/100", "IHCP-species-notes": "<p>Primary incubation for 30 mins at room temperature.</p>" } } }

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Product details

What is an oligoclonal antibody?
This oligoclonal antibody is a cocktail of monoclonal antibodies that have been carefully selected. Oligoclonal antibodies have not only the specificity and batch-to-batch consistency of a monoclonal antibody, but also have the advantage of the sensitivity of a polyclonal antibody due to their ability to recognize multiple epitopes on an antigen.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.

Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Pulmonary circulation 14:e70024 PubMed39678731

2024

Synchrotron-Based Phase-Contrast Micro-CT Combined With Histology to Decipher Differences Between Hereditary and Sporadic Pediatric Pulmonary Veno-Occlusive Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ida Jeremiasen,Niccolò Peruzzi,Elna Lampei,Sofie Meyer,Levent M Akyürek,Erik Gebre-Medhin,Ceren Mutgan,Peter Dorfmüller,Lavinia Neubert,Danny Jonigk,Csaba Galambos,Karin Tran-Lundmark

Nature communications 14:7710 PubMed38001067

2023

Deep topographic proteomics of a human brain tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Simon Davis,Connor Scott,Janina Oetjen,Philip D Charles,Benedikt M Kessler,Olaf Ansorge,Roman Fischer

eNeuro 9: PubMed36635242

2022

Localization of TRP Channels in Healthy Oral Mucosa from Human Donors.

Applications

Unspecified application

Species

Unspecified reactive species

Yalda Moayedi,Stephanie Michlig,Mark Park,Alia Koch,Ellen A Lumpkin

Journal of nanobiotechnology 20:481 PubMed36384529

2022

Bone marrow-targetable Green Tea Catechin-Based Micellar Nanocomplex for synergistic therapy of Acute myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ki Hyun Bae,Fritz Lai,Jamie Mong,Akiko Niibori-Nambu,Kiat Hwa Chan,Zhisheng Her,Motomi Osato,Min-Han Tan,Qingfeng Chen,Motoichi Kurisawa

Cell reports. Medicine 2:100421 PubMed34604819

2021

Determinants of SARS-CoV-2 entry and replication in airway mucosal tissue and susceptibility in smokers.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuguhisa Nakayama,Ivan T Lee,Sizun Jiang,Matthias S Matter,Carol H Yan,Jonathan B Overdevest,Chien-Ting Wu,Yury Goltsev,Liang-Chun Shih,Chun-Kang Liao,Bokai Zhu,Yunhao Bai,Peter Lidsky,Yinghong Xiao,David Zarabanda,Angela Yang,Meena Easwaran,Christian M Schürch,Pauline Chu,Han Chen,Anna K Stalder,David R McIlwain,Nicole A Borchard,Phillip A Gall,Sachi S Dholakia,Wei Le,Le Xu,Chih-Jaan Tai,Te-Huei Yeh,Elizabeth Erickson-Direnzo,Jason M Duran,Kirsten D Mertz,Peter H Hwang,Jasmin D Haslbauer,Peter K Jackson,Thomas Menter,Raul Andino,Peter D Canoll,Adam S DeConde,Zara M Patel,Alexandar Tzankov,Garry P Nolan,Jayakar V Nayak

Bioactive materials 6:3947-3961 PubMed33937594

2021

Tuning gelatin-based hydrogel towards bioadhesive ocular tissue engineering applications.

Applications

Unspecified application

Species

Unspecified reactive species

Sina Sharifi,Mohammad Mirazul Islam,Hannah Sharifi,Rakibul Islam,Darrell Koza,Felisa Reyes-Ortega,David Alba-Molina,Per H Nilsson,Claes H Dohlman,Tom Eirik Mollnes,James Chodosh,Miguel Gonzalez-Andrades

Journal of hematology & oncology 10:162 PubMed28985760

2017

An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia.

Applications

IHC-P

Species

Mouse

Zhisheng Her,Kylie Su Mei Yong,Kathirvel Paramasivam,Wilson Wei Sheng Tan,Xue Ying Chan,Sue Yee Tan,Min Liu,Yong Fan,Yeh Ching Linn,Kam Man Hui,Uttam Surana,Qingfeng Chen

Cancer biology & therapy 16:438-49 PubMed25679067

2015

Efficacy and safety of the hypoxia-activated prodrug TH-302 in combination with gemcitabine and nab-paclitaxel in human tumor xenograft models of pancreatic cancer.

Applications

IHC

Species

Unspecified reactive species

Jessica D Sun,Qian Liu,Dharmendra Ahluwalia,Wenwu Li,Fanying Meng,Yan Wang,Deepthi Bhupathi,Ayesha S Ruprell,Charles P Hart

Journal of immunology (Baltimore, Md. : 1950) 192:3816-27 PubMed24639355

2014

CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration.

Applications

Unspecified application

Species

Mouse

Hideo Kohno,Tadao Maeda,Lindsay Perusek,Eric Pearlman,Akiko Maeda

Nature medicine 19:421-8 PubMed23475202

2013

Preventive and therapeutic effects of Smad7 on radiation-induced oral mucositis.

Applications

Unspecified application

Species

Human

Gangwen Han,Li Bian,Fulun Li,Ana Cotrim,Donna Wang,Jianbo Lu,Yu Deng,Gregory Bird,Anastasia Sowers,James B Mitchell,J Silvio Gutkind,Rui Zhao,David Raben,Peter ten Dijke,Yosef Refaeli,Qinghong Zhang,Xiao-Jing Wang

View all publications

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