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AB30446

Anti-CD45 antibody [YTH24.5]

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(8 Publications)

Rat Monoclonal CD45 antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Flow Cytometry - Anti-CD45 antibody [YTH24.5] (AB30446)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD45 antibody [YTH24.5] (AB30446)

Overlay histogram showing peripheral blood lymphocytes stained with ab30446 (red line). The cells were incubated with the antibody (ab30446, 1μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (Fc) (ab96971) at 1/250 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (ab18541, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed gating on peripheral blood lymphocytes.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

YTH24.5

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Immunoglobulin fraction prepared by ammonium sulphate precipitation.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:29306 PubMed40790149

2025

Target sequence of single cells captured by a polymeric microfluidic device.

Applications

Unspecified application

Species

Unspecified reactive species

Rintaro Oyama,Masataka Mori,Hiroki Matsumiya,Yukiko Nemoto,Natsumasa Nishizawa,Yohei Honda,Masatoshi Kanayama,Akihiro Taira,Taiji Kuwata,Masaru Takenaka,Koji Kuroda,Kazue Yoneda,Takashi Ohnaga,Motoyoshi Endo,Fumihiro Tanaka

Stem cells international 2019:5310202 PubMed31885614

2019

The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Yuan,Chunyou Lai,Lingling Wei,Tianhang Feng,Qinyan Yang,Tianying Zhang,Tao Lan,Yutong Yao,Guangming Xiang,Xiaolun Huang

Stem cell reviews and reports 16:181-185 PubMed31758373

2019

Identification of Human Very Small Embryonic like Stem Cells (VSELS) in Human Heart Tissue Among Young and Old Individuals.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed El-Helw,Lakshman Chelvarajan,Mohamed Abo-Aly,Mohanad Soliman,Greg Milburn,Autumn L Conger,Kenneth Campbell,Mariusz Z Ratajczak,Ahmed Abdel-Latif

Oncology letters 18:6397-6404 PubMed31807163

2019

Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement.

Applications

Unspecified application

Species

Unspecified reactive species

Rei Ishibashi,Shuntaro Yoshida,Nariaki Odawara,Takahiro Kishikawa,Ryo Kondo,Ayako Nakada,Ryunosuke Hakuta,Naminatsu Takahara,Eri Tanaka,Kazuma Sekiba,Takahiro Seimiya,Takashi Ohnaga,Motoyuki Otsuka,Kazuhiko Koike

Stem cell research & therapy 10:225 PubMed31358049

2019

Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Yao,Ran Chen,Guowu Wang,Yu Zhang,Fang Liu

The Journal of experimental medicine 211:1925-35 PubMed25180064

2014

Niche displacement of human leukemic stem cells uniquely allows their competitive replacement with healthy HSPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Allison L Boyd,Clinton J V Campbell,Claudia I Hopkins,Aline Fiebig-Comyn,Jennifer Russell,Jelena Ulemek,Ronan Foley,Brian Leber,Anargyros Xenocostas,Tony J Collins,Mickie Bhatia

Proceedings of the National Academy of Sciences of 110:E4978-86 PubMed24297935

2013

Ascites analysis by a microfluidic chip allows tumor-cell profiling.

Applications

Unspecified application

Species

Human

Vanessa M Peterson,Cesar M Castro,Jaehoon Chung,Nathan C Miller,Adeeti V Ullal,Maria D Castano,Richard T Penson,Hakho Lee,Michael J Birrer,Ralph Weissleder

Cancer 80:2425-30 PubMed9406693

1997

Processing of antibodies bound to B-cell lymphomas and lymphoblastoid cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

N Vangeepuram,G L Ong,M J Mattes
View all publications
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