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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Mouse Monoclonal CD45 antibody. Suitable for IP, Flow Cyt, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 6 publications.
IgG2a
Mouse
pH: 8.2
Constituents: 100% Borate buffered saline
Liquid
Monoclonal
IP | Flow Cyt | IHC-FoFr | ICC/IF | |
---|---|---|---|---|
Mouse | Expected | Tested | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity.
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA, Ly-5, Ly-5, Ptprc
Mouse Monoclonal CD45 antibody. Suitable for IP, Flow Cyt, IHC-FoFr, ICC/IF and reacts with Mouse samples. Cited in 6 publications.
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA, Ly-5, Ly-5, Ptprc
IgG2a
Mouse
pH: 8.2
Constituents: 100% Borate buffered saline
Liquid
Monoclonal
A20
Affinity purification
kappa
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
ab25078 recognizes all mouse leukocytes expressing the CD45.1 allotype and is useful as a marker for origin of donor and host cells.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.
CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.
CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.
CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/106 cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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