AB25078

Anti-CD45.1 antibody [A20]

Name: Anti-CD45.1 antibody [A20] (ab25078) | Abcam
Brand: Abcam Limited
SKU: AB25078
Price: 520 USD
Availability: InStock

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(7 Publications)

Anti-CD45.1 antibody [A20] (ab25078) is a mouse monoclonal antibody detecting CD45.1 in Flow Cytometry, IP, ICC/IF. Suitable for Mouse.

- Trusted since 2005

View Alternative Names

CD45, Ly-5, Ptprc, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, Lymphocyte antigen 5, T200, L-CA

1 Images

Flow Cytometry - Anti-CD45.1 antibody [A20] (AB25078)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45.1 antibody [A20] (AB25078)

Flow Cytometry analysis of SJL mouse splenocytes labeling CD45.1 with ab25078 at 0.1 μg/10⁶ cells (Purple). A Goat Anti-Mouse IgG2a, Human ads-AF647 was used as the secondary antibody. Grey - Isotype Control, Mouse IgG2a-UNLB followed by Goat Anti-Mouse IgG2a, Human ads-AF647.

667 Cy5®

Cy5 ® Anti-CD45.1 antibody [A20]
660 APC

APC Anti-CD45.1 antibody [A20]
519 FITC

FITC Anti-CD45.1 antibody [A20]
Biotin

Biotin Anti-CD45.1 antibody [A20]
667 PE/Cy5®

PE/Cy5® Anti-CD45.1 antibody [A20]
695 PerCP/Cy5.5®

PerCP/Cy5.5® Anti-CD45.1 antibody [A20]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A20

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

ICC/IF, Flow Cyt, IHC-FoFr, IP

applications

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Reactivity data

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Product details

What is this antibody validated in?
Anti-CD45.1 antibody [A20] (ab25078) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Other related products
We have a range of other formats of antibody clone [A20] also available for your convenience: ab210186, ab210187, FITC - ab24917, Cy5® - ab24982, Biotin - ab25011

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 8.2 Constituents: 100% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45.1 also known as Leukocyte Common Antigen (LCA) or Protein Tyrosine Phosphatase Receptor Type C (PTPRC) is a protein expressed on the surface of all nucleated hematopoietic cells. It has a molecular mass of approximately 180-220 kDa. This protein acts as a receptor-type protein tyrosine phosphatase. CD45.1 is an essential marker in the immune system particularly found on T cells B cells and other white blood cells. The expression of CD45.1 is seen across leukocytes where it plays a significant role in cell signaling and antigen receptor signaling.

Biological function summary

CD45.1 influences the immune response by modulating signals through antigen receptors. It operates as a single-pass type I membrane protein and is not known to be part of a larger complex. This protein is important in the activation and regulation of T-cells and B-cells by fine-tuning the threshold of signals required for cell activation. The presence of CD45.1 affects multiple processes in adaptive immunity impacting lymphocyte development and their further activation in immune responses.

Pathways

CD45.1 is integral in lymphocyte activation pathways. It influences the T-cell receptor (TCR) and B-cell receptor (BCR) signaling pathways by dephosphorylating specific kinases like Lck and Fyn which are important in initiating TCR and BCR signaling cascades. Moreover CD45.1 interacts with other proteins such as CD3 and CD19 being essential in the regulation of immune response and maintaining proper signaling amplitudes to ensure effective immune function.

CD45.1 is intricately linked to autoimmune diseases and leukemia. The protein's dysregulation can lead to improper immune signaling contributing to conditions such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). In leukemia the altered expression of CD45.1 and related proteins such as CD20 often correlates with disease progression and treatment response. Understanding the roles of CD45.1 can facilitate the development of targeted therapies in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity. Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.

See full target information Ptprc

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed38888968

2024

Dysfunction of infiltrating cytotoxic CD8+ T cells within the graft promotes murine kidney allotransplant tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Takahiro Yokose,Edward S Szuter,Ivy Rosales,Michael T Guinn,Andrew S Liss,Taisuke Baba,David A Ruddy,Michelle Piquet,Jamil Azzi,A Benedict Cosimi,Paul S Russell,Joren C Madsen,Robert B Colvin,Alessandro Alessandrini

Cellular and molecular gastroenterology and hepatology 15:613-632 PubMed36436756

2022

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qianjin Zhang,Xiaomin Su,Chunze Zhang,Wei Chen,Ya Wang,Xiaorong Yang,Dan Liu,Yuan Zhang,Rongcun Yang

Experimental and therapeutic medicine 20:375-384 PubMed32509014

2020

Modulation of the secretion of mesenchymal stem cell immunoregulatory factors by hydrolyzed fish collagen.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Jiao Sun

Oncoimmunology 5:e1211218 PubMed27757303

2016

Efficient tumor regression by adoptively transferred CEA-specific CAR-T cells associated with symptoms of mild cytokine release syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Linan Wang,Ning Ma,Sachiko Okamoto,Yasunori Amaishi,Eiichi Sato,Naohiro Seo,Junichi Mineno,Kazutoh Takesako,Takuma Kato,Hiroshi Shiku

EMBO molecular medicine 5:471-81 PubMed23417922

2013

Distinct functions of chemokine receptor axes in the atherogenic mobilization and recruitment of classical monocytes.

Applications

IHC-Fr

Species

Mouse

Oliver Soehnlein,Maik Drechsler,Yvonne Döring,Dirk Lievens,Helene Hartwig,Klaus Kemmerich,Almudena Ortega-Gómez,Manuela Mandl,Santosh Vijayan,Delia Projahn,Christoph D Garlichs,Rory R Koenen,Mihail Hristov,Esther Lutgens,Alma Zernecke,Christian Weber

PloS one 4:e5728 PubMed19478957

2009

A non-cytosolic protein of Trypanosoma evansi induces CD45-dependent lymphocyte death.

Applications

Unspecified application

Species

Mouse

Nicolas Antoine-Moussiaux,Anne Cornet,François Cornet,Stéphanie Glineur,Martin Dermine,Daniel Desmecht

Journal of virology 81:9801-11 PubMed17626103

2007

CD40-CD40 ligand interactions promote trafficking of CD8+ T cells into the brain and protection against West Nile virus encephalitis.

Applications

IHC-FoFr

Species

Mouse

Elizabeth Sitati,Erin E McCandless,Robyn S Klein,Michael S Diamond

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD45.1 antibody [A20]

Flow Cytometry - Anti-CD45.1 antibody [A20] (AB25078)

Related conjugates and formulations (6)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise