AB755

Anti-CD45RA antibody [4KB5]

BOND RX™ Validated
Recombinant
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Mouse Recombinant Monoclonal CD45 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 7 publications.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images

Flow Cytometry - Anti-CD45RA antibody [4KB5] (AB755)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RA antibody [4KB5] (AB755)

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) (right) labeling CD45RA with ab755 at 1.017μg/ml compared with mouse monoclonal IgG Isotype Control (left). Goat anti-mouse IgG (Alexa Fluor^® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (AB755)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (AB755)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD45RA with ab755 at 0.239μg/ml, followed by ready to use secondary. Positive staining on human tonsil tissue is observed. The section was incubated with ab755 for 10 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use secondary.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Carrier free

Anti-CD45RA antibody [4KB5] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4KB5

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.017 µg/mL", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.239 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RA also known as CD45RA antigen and T200 is an isoform of the CD45 protein family. It is a transmembrane protein tyrosine phosphatase with a molecular mass of approximately 220 kDa. CD45RA expression is abundant on naive T-cells and subsets of other immune cells like B cells. Researchers frequently use CD45RA antibodies including CD45RA-PE and CD45RA-FITC for flow cytometry to identify and study immune cell subsets.

Biological function summary

CD45RA plays a significant role in regulating T-cell receptor (TCR) signaling. By dephosphorylating specific phosphotyrosine residues on substrates CD45RA modulates signal transduction pathways important for T-cell activation and differentiation. This protein does not act alone but is part of a regulatory network that influences immune responses and cellular development through its interaction with other cell surface receptors and intracellular kinases.

Pathways

CD45RA modulation is integral to T-cell development and activation processes. It is an important participant in the T-cell receptor signaling pathway and the Jak-STAT signaling pathway. In these pathways CD45 interacts with proteins such as Lck and Fyn which are essential for propagating receptor-mediated signaling events in T-cells. Effective signal regulation through CD45RA is necessary to ensure appropriate immune responses.

CD45RA has links to autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The aberrant expression or function of CD45RA on immune cells can lead to improper immune activation or suppression contributing to the pathology of these diseases. Crossover with other proteins such as CD45RO which is an isoform involved in memory T-cell function indicates a broader network of immune regulation where CD45RA maintains the balance between immune activation and tolerance.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:25270 PubMed40652059

2025

Myeloperoxidase expressing tumor associated neutrophils are associated with worse prognosis in metastatic breast cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Karin Leandersson,Dag Blomgård,Oscar Tuvesson,Lisa Rydén,Caroline Bergenfelz,Anna-Maria Larsson

Cellular & molecular immunology 22:316-329 PubMed39939817

2025

Uncovering two neutrophil-committed progenitors that immediately precede promyelocytes during human neutropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Signoretto,Federica Calzetti,Giulia Finotti,Silvia Lonardi,Camillo Balanzin,Francisco Bianchetto-Aguilera,Sara Gasperini,Elisa Gardiman,Monica Castellucci,Anna Russignan,Massimiliano Bonifacio,Antonio Sica,William Vermi,Cristina Tecchio,Patrizia Scapini,Nicola Tamassia,Marco A Cassatella

Journal of translational medicine 23:61 PubMed39806464

2025

The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Yan Yang,Nian Chen,Qian Wen,Yu Zhou,Tao Zhang,Ji Zhou,Cheng-Hui Liang,Li-Ping Han,Xiao-Ya Wang,Qing-Mei Kang,Xiao-Xia Zheng,Xue-Jia Zhai,Hong-Ying Jiang,Tian-Hua Shen,Jin-Wei Xiao,Yu-Xin Zou,Yun Deng,Shuang Lin,Jiang-Jie Duan,Jun Wang,Shi-Cang Yu

Frontiers in immunology 12:769047 PubMed34777388

2021

The Impact of Immune Microenvironment on the Prognosis of Pancreatic Ductal Adenocarcinoma Based on Multi-Omics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yang,Mingyao Zhou,Yunzi Wu,Yuanyuan Ma,Qin Tan,Wei Yuan,Jie Ma

Cell reports. Medicine 2:100392 PubMed34622229

2021

Molecular predictors of response to pembrolizumab in thymic carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yongfeng He,Archana Ramesh,Yuriy Gusev,Krithika Bhuvaneshwar,Giuseppe Giaccone

International journal of biological sciences 17:20-31 PubMed33390830

2021

ACE2 Correlated With Immune Infiltration Serves As A Novel Prognostic Biomarker In Clear Cell Renal Cell Carcinoma: Implication For COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Wuping Yang,Lei Li,Kenan Zhang,Kaifang Ma,Haibiao Xie,Yanqing Gong,Jingcheng Zhou,Kan Gong

Blood 105:3295-302 PubMed15626731

2005

A rapid translocation of CD45RO but not CD45RA to lipid rafts in IL-6-induced proliferation in myeloma.

Applications

Unspecified application

Species

Unspecified reactive species

Fu-Jun Li,Naohiro Tsuyama,Hideaki Ishikawa,Masanori Obata,Saeid Abroun,Shangqin Liu,Ken-ichiro Otsuyama,Xu Zheng,Zi Ma,Yasuko Maki,Michio M Kawano

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD45RA antibody [4KB5]

Flow Cytometry - Anti-CD45RA antibody [4KB5] (AB755)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [4KB5] (AB755)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise