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AB308425

Anti-CD45RA antibody [GT0014] - BSA and Azide free

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Mouse Monoclonal CD45 antibody. Carrier free. Suitable for IHC-P, Flow Cyt, ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PTPRC.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

5 Images
Flow Cytometry - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)

Flow cytometric analysis of THP-1 cell fixed in 4% paraformaldehyde at 4C for 15 minutes labelling CD45RA with ab308425 at 1/100 dilution (green) compared with a Isotype control at 1/100 dilution (black).

Flow Cytometry - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)

Flow cytometric analysis of THP-1 cells labelling CD45RA with ab308425 at 1/50 dilution (red) compared with a unlabeled control (black). Acquisition of 20,000 events were collected for FACS analysis

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)

Immunohistochemical analysis of paraffin-embedded human tonsil and placenta tissues stained for CD45RA protein with ab308425 at a 1/200 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min. Corresponding RNA levels (nTPM) in the tissues are based on Human Protein Atlas program.

Immunocytochemistry/ Immunofluorescence - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)

For immunofluorescence analysis, Raji cells were fixed in ice-cold MeOH for 5 minutes and permeabilized for detection of CD45RA protein using ab308425 at 1/500 dilution (green). Blue : Hoechst 33342 staining.

Western blot - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)
  • WB

Supplier Data

Western blot - Anti-CD45RA antibody [GT0014] - BSA and Azide free (AB308425)

Raji whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45RA antibody [GT0014] (ab308425) diluted at 1/1000. The observed M.W. is based on the publication : PMID : 24405902

All lanes:

Western blot - Anti-CD45RA antibody [GT0014] - BSA and Azide free (ab308425) at 1/1000 dilution

Lane 1:

Raji cell lysate at 30 µg

Lane 2:

Raji membrane extract at 30 µg

Predicted band size: 147.5 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

GT0014

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PTPRC. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50 - 1/200", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Product details

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45 also known as Protein tyrosine phosphatase receptor type C (PTPRC) is a transmembrane glycoprotein with a molecular mass ranging between 180-240 kDa depending on its isoform. It is expressed on the surface of almost all hematopoietic cells except for mature erythrocytes and platelets. CD45 has a critical role in regulating antigen receptor signaling by modifying kinases involved in signal transduction and it is essential in lymphocyte development and activation. Because of its broad expression on immune cells CD45 is a valuable marker for differentiating various immune cell types in assays like flow cytometry and immunohistochemistry (IHC) often referred to as CD45 stain.
Biological function summary

CD45 acts by regulating tyrosine phosphorylation in the immune cell signaling context participating directly in signal transduction. It functions by dephosphorylating specific phosphotyrosine residues on various proteins modulating the signaling threshold required for lymphocyte activation. Although not known to be part of a protein complex CD45 itself shows isoform variation that associates with specific immune cell types impacting their function. Importantly CD45 interacts with multiple signaling molecules to affect cell growth and differentiation.

Pathways

Scientists associate CD45 with signaling pathways such as the T-cell receptor (TCR) and B-cell receptor (BCR) signaling. CD45 modulates the activity of Src family kinases important elements in these pathways making it essential for effective immune response and tolerance. Furthermore interactions between CD45 and proteins like Lck in T-cells or Lyn in B-cells highlight its pivotal role in executing its signaling functions.

Scientists often link CD45 to immune-related conditions including autoimmunity and leukemia. In autoimmune diseases altered CD45 expression or activity can disrupt normal immune function contributing to pathogenesis. In leukemia CD45 expression levels can assist in disease classification and prognosis as it interacts with proteins involved in cell cycle regulation. The anti-CD45 antibodies can provide diagnostic and therapeutic avenues highlighting their utility in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Product promise

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