AB8217

Anti-CD45RA antibody [MEM-56]

Name: Anti-CD45RA antibody [MEM-56] (ab8217) | Abcam
Brand: Abcam Limited
SKU: AB8217
Price: 545 USD
Availability: InStock

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(4 Publications)

Mouse Monoclonal CD45 antibody. Suitable for WB, IHC-P, Flow Cyt and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (AB8217)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (AB8217)

Immunohistochemical analysis of Paraffin-embedded sections Human Tonsil tissue labelling CD45RA with ab8217 at 5µg/ml.

Flow Cytometry - Anti-CD45RA antibody [MEM-56] (AB8217)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RA antibody [MEM-56] (AB8217)

Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RA with ab8217 at 0.3 ug/ml showing separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

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Western blot - Anti-CD45RA antibody [MEM-56] (AB8217)

Lane 1:

CD45 control antibody

Lanes 2 - 3:

Western blot - Anti-CD45RA antibody [MEM-56] (ab8217)

Lane 1:

HUT-78 human cutaneous T cell lymphoma cell line.

Lane 2:

sample of cell lysate prepared under non-reducing conditions

Lane 3:

sample of cell lysate prepared under reducing conditions

Predicted band size: 147 kDa

false

519 FITC

FITC Anti-CD45RA antibody [MEM-56]
618 PE/DyLight™ 594

PE/DyLight™ 594 Anti-CD45RA antibody [MEM-56]
778 PE/Cy7®

PE/Cy7® Anti-CD45RA antibody [MEM-56]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-56

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Cross reacts with an intracellular 60 kDa protein of unknown identity like other anti-CD45RA MAbs.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "No pre-treatment of tissue sections is needed.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.3-4 µg/mL", "FlowCyt-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from TCS

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RA also known as CD45RA antigen and T200 is an isoform of the CD45 protein family. It is a transmembrane protein tyrosine phosphatase with a molecular mass of approximately 220 kDa. CD45RA expression is abundant on naive T-cells and subsets of other immune cells like B cells. Researchers frequently use CD45RA antibodies including CD45RA-PE and CD45RA-FITC for flow cytometry to identify and study immune cell subsets.

Biological function summary

CD45RA plays a significant role in regulating T-cell receptor (TCR) signaling. By dephosphorylating specific phosphotyrosine residues on substrates CD45RA modulates signal transduction pathways important for T-cell activation and differentiation. This protein does not act alone but is part of a regulatory network that influences immune responses and cellular development through its interaction with other cell surface receptors and intracellular kinases.

Pathways

CD45RA modulation is integral to T-cell development and activation processes. It is an important participant in the T-cell receptor signaling pathway and the Jak-STAT signaling pathway. In these pathways CD45 interacts with proteins such as Lck and Fyn which are essential for propagating receptor-mediated signaling events in T-cells. Effective signal regulation through CD45RA is necessary to ensure appropriate immune responses.

CD45RA has links to autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. The aberrant expression or function of CD45RA on immune cells can lead to improper immune activation or suppression contributing to the pathology of these diseases. Crossover with other proteins such as CD45RO which is an isoform involved in memory T-cell function indicates a broader network of immune regulation where CD45RA maintains the balance between immune activation and tolerance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PloS one 10:e0132617 PubMed26147970

2015

The Macrophage Galactose-Type C-Type Lectin (MGL) Modulates Regulatory T Cell Functions.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Grazia Zizzari,Paola Martufi,Federico Battisti,Hassan Rahimi,Salvatore Caponnetto,Filippo Bellati,Marianna Nuti,Aurelia Rughetti,Chiara Napoletano

BMC immunology 9:73 PubMed19087256

2008

Changing survival, memory cell compartment, and T-helper balance of lymphocytes between severe and mild asthma.

Applications

ICC

Species

Human

A S Abdulamir,R R Hafidh,F Abubakar,K A Abbas

Immunogenetics 29:202-5 PubMed2466778

1989

Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Hilgert,H Kristofová,D Maurer,V Horejsí

Folia biologica 35:289-97 PubMed2534480

1989

Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Stefanová,I Hilgert,H Kristofová,S Vanĕk,A Bukovský,V Horejsí

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD45RA antibody [MEM-56]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RA antibody [MEM-56] (AB8217)

Flow Cytometry - Anti-CD45RA antibody [MEM-56] (AB8217)

Western blot - Anti-CD45RA antibody [MEM-56] (AB8217)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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