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AB8218

Anti-CD45RB antibody [MEM-55]

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(2 Publications)

Mouse Monoclonal CD45 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RB antibody [MEM-55] (AB8218)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RB antibody [MEM-55] (AB8218)

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded human tonsil tissue sections with ab8218.

Flow Cytometry - Anti-CD45RB antibody [MEM-55] (AB8218)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RB antibody [MEM-55] (AB8218)

Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RB with ab8218 at 1 ug/ml showing separation of human CD45RB positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-55

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

Specificity

CD45RB antigen (human). This antibody recognizes a siliadase-sensitive epitope expressed on most PBL.

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Alternative Products

Primary Antibodies

AB218504

Anti-CD45RB antibody [PTPRC/1783R]

Recombinant

Flow Cytometry - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

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Primary Antibodies

AB194400

PE Anti-CD45RB antibody [BRA-11]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PE Anti-CD45RB antibody [BRA-11] (AB194400)

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Primary Antibodies

AB187567

Alexa Fluor® 647 Anti-CD45RB antibody [MEM55]

Flow Cytometry - Alexa Fluor® 647 Anti-CD45RB antibody [MEM55] (AB187567)

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Primary Antibodies

AB25647

PE Anti-CD45RB antibody [C363.16A]

Flow Cytometry - PE Anti-CD45RB antibody [C363.16A] (AB25647)

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Complementary Products

Assay Kits

AB277445

Mouse CD45 ELISA kit

ELISA - Mouse CD45 ELISA kit (AB277445)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

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Anti-CD3 epsilon antibody [SP7]

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Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

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Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

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Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>No pre-treatment of tissue sections is needed.</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.5-2 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from TCS
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RB also known as protein tyrosine phosphatase receptor type C (PTPRC) is a membrane-associated protein found prominently on all nucleated hematopoietic cell surfaces. This protein has a molecular mass that ranges between 180-220 kDa depending on the variable splicing. Importantly CD45RB is a component of the CD45 group playing an important role in regulating antigen receptor signaling in lymphocytes affecting both T-cell and B-cell function. Its expression occurs mainly in hematopoietic tissues such as the bone marrow lymph nodes and spleen.
Biological function summary

CD45RB functions as an integral transmembrane protein-tyrosine phosphatase and does not operate as part of a complex but rather acts directly on its substrates. It regulates the phosphorylation state of Src family kinases that are necessary for antigen receptor signaling. By modulating this phosphorylation CD45RB controls signal transduction influencing growth differentiation and immune response in lymphocytes. Its regulatory capacity extends to alterations in cell cycle progression and apoptosis maintaining homeostasis within the immune system.

Pathways

Studies link CD45RB to important pathways like the T-cell receptor signaling and B-cell receptor signaling pathways. These pathways are essential for the activation and differentiation of these immune cells. CD45RB acts in coordination with kinases such as Lck and Fyn (of the Src family) aiding in the dephosphorylation required for their activation. This interaction highlights CD45RB's fundamental role in fine-tuning responses to external antigens enabling appropriate immune responses.

CD45RB has associations particularly with autoimmune diseases and leukemia. Aberrant expression or function of CD45RB can lead to improper immune function contributing to conditions like multiple sclerosis and certain leukemias. Interactions with other membrane proteins including BRCA1 can also indirectly influence cancer dynamics through immune surveillance mechanisms. These associations make CD45RB a target of interest in developing therapeutic strategies for immune-dysregulation and hematological malignancies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Immunogenetics 29:202-5 PubMed2466778

1989

Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.

Applications

Unspecified application

Species

Unspecified reactive species

V Bazil,I Hilgert,H Kristofová,D Maurer,V Horejsí

Folia biologica 34:23-34 PubMed2968928

1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I Hilgert
View all publications
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