Mouse Monoclonal CD45 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.
Images
![Flow Cytometry - Anti-CD45RB antibody [MEM-55] (AB8218), expandable thumbnail](https://content.abcam.com/products/images/cd45rb-antibody-mem-55-ab8218--flow-cytometry-img422656.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD45RB antibody [MEM-55] (AB8218)")
Publications
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- Immunogenetics 29:202-51989Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesV Bazil,I Hilgert,H Kristofová,D Maurer,V HorejsíPubMed 2466778
- Folia biologica 34:23-341988Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).Applications:Unspecified applicationReactive species:Unspecified reactive speciesV Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I HilgertPubMed 2968928
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
Reactivity data
Select an application| IHC-P | Flow Cyt | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes No pre-treatment of tissue sections is needed. Antigen retrieval is not essential but may optimise staining. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-2 µg/mL | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Alternative names
CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA
Recommended products
Mouse Monoclonal CD45 antibody. Suitable for IHC-P, Flow Cyt and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
- Clone number
- MEM-55
- Purification technique
- Affinity purification Protein A
- Specificity
CD45RB antigen (human). This antibody recognizes a siliadase-sensitive epitope expressed on most PBL.
- Concentration
- Purification notes
Purified from TCS
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD45RB also known as protein tyrosine phosphatase receptor type C (PTPRC) is a membrane-associated protein found prominently on all nucleated hematopoietic cell surfaces. This protein has a molecular mass that ranges between 180-220 kDa depending on the variable splicing. Importantly CD45RB is a component of the CD45 group playing an important role in regulating antigen receptor signaling in lymphocytes affecting both T-cell and B-cell function. Its expression occurs mainly in hematopoietic tissues such as the bone marrow lymph nodes and spleen.
- Biological function summary
CD45RB functions as an integral transmembrane protein-tyrosine phosphatase and does not operate as part of a complex but rather acts directly on its substrates. It regulates the phosphorylation state of Src family kinases that are necessary for antigen receptor signaling. By modulating this phosphorylation CD45RB controls signal transduction influencing growth differentiation and immune response in lymphocytes. Its regulatory capacity extends to alterations in cell cycle progression and apoptosis maintaining homeostasis within the immune system.
- Pathways
Studies link CD45RB to important pathways like the T-cell receptor signaling and B-cell receptor signaling pathways. These pathways are essential for the activation and differentiation of these immune cells. CD45RB acts in coordination with kinases such as Lck and Fyn (of the Src family) aiding in the dephosphorylation required for their activation. This interaction highlights CD45RB's fundamental role in fine-tuning responses to external antigens enabling appropriate immune responses.
- Associated diseases and disorders
CD45RB has associations particularly with autoimmune diseases and leukemia. Aberrant expression or function of CD45RB can lead to improper immune function contributing to conditions like multiple sclerosis and certain leukemias. Interactions with other membrane proteins including BRCA1 can also indirectly influence cancer dynamics through immune surveillance mechanisms. These associations make CD45RB a target of interest in developing therapeutic strategies for immune-dysregulation and hematological malignancies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Flow Cytometry - Anti-CD45RB antibody [MEM-55] (ab8218), expandable thumbnail](https://content.abcam.com/products/images/cd45rb-antibody-mem-55-ab8218--flow-cytometry-img422656.jpg "Flow Cytometry - Anti-CD45RB antibody [MEM-55] (ab8218)")
Flow Cytometry - Anti-CD45RB antibody [MEM-55] (ab8218)
Flow cytometric analysis of Human Peripheral Blood cells labelling CD45RB with ab8218 at 1 ug/ml showing separation of human CD45RB positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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