AB218504

Anti-CD45RB antibody [PTPRC/1783R]

Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD45 antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

4 Images

Flow Cytometry - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

Flow Cytometry analysis of PFA-fixed Raji (human Burkitt's lymphoma cell line) cells labeling CD45RB with ab218504 at 2 μg/10⁶ cells followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).

Supplier Data

Immunocytochemistry - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

Immunocytochemistry analysis of PFA-fixed Raji (human Burkitt's lymphoma cell line) cells labeling CD45RB with ab218504 at 2 μg/mL followed by goat anti-rabbit IgG-CF488 (green). Counterstain is phalloidin (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD45RB with ab218504 at 1 μg/mL for 30 minutes at RT.

Supplier Data

Western blot - Anti-CD45RB antibody [PTPRC/1783R] (AB218504)

All lanes:

Western blot - Anti-CD45RB antibody [PTPRC/1783R] (ab218504) at 2 µg/mL

All lanes:

Human spleen tissue lysate

Predicted band size: 147 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

PTPRC/1783R

Isotype

IgG

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC, Flow Cyt

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RB also known as protein tyrosine phosphatase receptor type C (PTPRC) is a membrane-associated protein found prominently on all nucleated hematopoietic cell surfaces. This protein has a molecular mass that ranges between 180-220 kDa depending on the variable splicing. Importantly CD45RB is a component of the CD45 group playing an important role in regulating antigen receptor signaling in lymphocytes affecting both T-cell and B-cell function. Its expression occurs mainly in hematopoietic tissues such as the bone marrow lymph nodes and spleen.

Biological function summary

CD45RB functions as an integral transmembrane protein-tyrosine phosphatase and does not operate as part of a complex but rather acts directly on its substrates. It regulates the phosphorylation state of Src family kinases that are necessary for antigen receptor signaling. By modulating this phosphorylation CD45RB controls signal transduction influencing growth differentiation and immune response in lymphocytes. Its regulatory capacity extends to alterations in cell cycle progression and apoptosis maintaining homeostasis within the immune system.

Pathways

Studies link CD45RB to important pathways like the T-cell receptor signaling and B-cell receptor signaling pathways. These pathways are essential for the activation and differentiation of these immune cells. CD45RB acts in coordination with kinases such as Lck and Fyn (of the Src family) aiding in the dephosphorylation required for their activation. This interaction highlights CD45RB's fundamental role in fine-tuning responses to external antigens enabling appropriate immune responses.

CD45RB has associations particularly with autoimmune diseases and leukemia. Aberrant expression or function of CD45RB can lead to improper immune function contributing to conditions like multiple sclerosis and certain leukemias. Interactions with other membrane proteins including BRCA1 can also indirectly influence cancer dynamics through immune surveillance mechanisms. These associations make CD45RB a target of interest in developing therapeutic strategies for immune-dysregulation and hematological malignancies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). Interacts with CLEC10A at antigen presenting cell-T cell contact; CLEC10A on immature dendritic cells recognizes Tn antigen-carrying PTPRC/CD45 receptor on effector T cells and modulates T cell activation threshold to limit autoreactivity.. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.

See full target information PTPRC

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39769197

2025

Investigation of -Acetyllactosamine and ,-Diacetyllactosamine Residues of Seminal Plasma Prolactin-Induced Protein as Ligands Recognized by Galectin-3.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Kałuża,Katarzyna Trzęsicka,Damian Drzyzga,Mirosława Ferens-Sieczkowska

View all publications

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Product promise

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