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AB244570

Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free

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(1 Publication)

Mouse Monoclonal CD45 antibody. Carrier free. Suitable for IHC-Fr, IHC-P, Flow Cyt and reacts with Rat, Human samples. Cited in 1 publication.

View Alternative Names

CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA, Ptprc

1 Images
Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (AB244570)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD45RC antibody [MRC OX-22] - BSA and Azide free (AB244570)

IHC image of CD45RC staining in a section of frozen normal rat spleen*.

The section was fixed using 10% formaldehyde in 1XPBS for 10 minutes. No antigen retrieval step was performed prior to staining.

Non-specific protein-protein interactions were blocked using TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 3% (w/v) BSA for 1 hour at room temperature. The section was then incubated with ab33945 (1µg/ml dilution) in TBS containing 0.025% (v/v) Triton X-100 and 3% (w/v) BSA overnight at +4°C. The section was then incubated with ab150119 ((Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647) (shown in red) and DAPI (staining nuclear DNA) (shown in blue) for 1 hour at room temperature. The section was then mounted using Fluoromount®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

The DAPI only control (no antibody) inset shows no autofluorescence, demonstrating that any Alexa Fluor® 647 signal is derived directly from bound ab33945.

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from Charles River.

This data was developed using the same antibody clone in a different buffer formulation containing PBS and Sodium Azide (ab33945)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MRC OX-22

Isotype

IgG1

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min).</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1 µg/mL", "IHCFr-species-notes": "<p>This product gave a positive signal in HeLa cells fixed with 10% formaldehyde (10 min).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab244570 is the carrier-free version of ab33945.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RC also known as PTPRC isoform 14125 or CD45 plays an essential mechanical role as a protein tyrosine phosphatase. This approximately 180-240 kDa transmembrane protein modulates antigen receptor signaling in lymphocytes. CD45RC is expressed on various immune cells including T cells B cells and natural killer cells with expression levels varying between subsets of these cells. The CD45RC isoform arises from alternative splicing leading to different molecular forms that can affect immune cell signaling.
Biological function summary

CD45RC impacts cell signaling by regulating the threshold of activation in T and B cells. It is a member of the receptor-type protein tyrosine phosphatase family and functions to maintain immune homeostasis. CD45RC does not typically function alone; it interacts with other cell surface molecules to facilitate signal transduction. By dephosphorylating specific kinases it fine-tunes the immune response preventing overreaction and facilitating a rapid appropriate response to antigens.

Pathways

CD45RC modulates pathways involved in lymphocyte activation. One key pathway is the T-cell receptor signaling pathway where it controls the activity of Src family kinases which play a significant part in the early activation of T cells. Another important pathway is the B-cell receptor signaling pathway. CD45RC's action with the BCR complex ensures precise regulation of B cell responses. Its activity is closely related to proteins such as Lck and Fyn which are involved in the signaling cascades initiated by receptor engagement.

CD45RC has implications in autoimmune diseases and certain leukemias. Its altered expression or function can lead to improper immune system regulation which may contribute to autoimmune conditions like rheumatoid arthritis where an overactive immune response targets the body’s own tissues. In some leukemias aberrant CD45RC expression can affect leukemia progression by modulating leukemia cell signaling and interactions within the bone marrow niche. This relationship of CD45RC with disease-related proteins like Lck highlights its potential as a therapeutic target in conditions of immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN (By similarity). Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).
See full target information Ptprc
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 158:795-810 PubMed6224884

1983

MRC OX-22, a monoclonal antibody that labels a new subset of T lymphocytes and reacts with the high molecular weight form of the leukocyte-common antigen.

Applications

Unspecified application

Species

Unspecified reactive species

G P Spickett,M R Brandon,D W Mason,A F Williams,G R Woollett
View all publications
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