Mouse Monoclonal CD45 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (AB212786), expandable thumbnail](https://content.abcam.com/products/images/cd45ro-antibody-t200-797-bsa-and-azide-free-ab212786--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130219.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (AB212786)")
Publications
Filter by
- Frontiers in immunology 14:11626692023Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo VilarPubMed 37207208
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-0.50000 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed:35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Alternative names
CD45, PTPRC, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA
Recommended products
Mouse Monoclonal CD45 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing PTPRC protein.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information. Database link P08575
- Carrier free
- Yes
- Clone number
- T200/797
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab212786 is the carrier-free version of Anti-CD45RO antibody [T200/797] ab216024.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CD45RO also known as leukocyte common antigen 2 is an important isoform of the protein tyrosine phosphatase receptor type C (PTPRC). This protein has a molecular mass of about 180 kDa. Researchers find CD45RO expressed mostly on memory T cells a subset of T lymphocytes. These cells play an essential role in the immune system by remembering past infections or vaccinations. CD45RO functions mechanistically by modulating T cell receptor (TCR) signaling which is important for antibody production.
- Biological function summary
This isoform plays an important role in immune responses by supporting the activation and differentiation of T cells. It is not part of a larger protein complex itself but interacts with other immune cell surface proteins for effective signaling. CD45RO is particularly important in the modulation of signal transduction through its phosphatase activity which removes phosphate groups from specific tyrosine residues on proteins influencing T cell sensitivity and response to antigens.
- Pathways
CD45RO influences the JAK/STAT pathway a critical signaling pathway for cytokine receptor signaling in immune cells. It also plays a role in the TCR signaling pathway which is responsible for T cell activation. Within these pathways CD45RO acts in conjunction with other proteins like Lck and ZAP-70 that are also integral parts of T cell activation and proliferation processes essential for adaptive immune responses.
- Associated diseases and disorders
CD45RO is associated with autoimmune disorders such as rheumatoid arthritis where memory T cells play a role in sustaining inflammation. CD45RO also relates to chronic hepatitis B infection as it affects the disease progression through memory T cell-mediated immune responses. In these contexts CD45RO's interaction with other proteins like HLA-DR which is another immune-related surface protein becomes significant as it helps modulate immune responses that either suppress or exacerbate the disease symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (ab212786), expandable thumbnail](https://content.abcam.com/products/images/cd45ro-antibody-t200-797-bsa-and-azide-free-ab212786--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130219.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (ab212786)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (ab212786)
Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue labeling CD45RO with ab212786 at 0.5 µg/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com