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AB212786

Anti-CD45RO antibody [T200/797] - BSA and Azide free

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(2 Publications)

Mouse Monoclonal CD45 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing PTPRC protein.

View Alternative Names

CD45, Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, T200, L-CA

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (AB212786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [T200/797] - BSA and Azide free (AB212786)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human tonsil tissue labeling CD45RO with ab212786 at 0.5 µg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

T200/797

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Cell preparation containing PTPRC protein. The exact immunogen used to generate this antibody is proprietary information.

P08575

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.25-0.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

ab212786 is the carrier-free version of ab216024.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD45RO also known as leukocyte common antigen 2 is an important isoform of the protein tyrosine phosphatase receptor type C (PTPRC). This protein has a molecular mass of about 180 kDa. Researchers find CD45RO expressed mostly on memory T cells a subset of T lymphocytes. These cells play an essential role in the immune system by remembering past infections or vaccinations. CD45RO functions mechanistically by modulating T cell receptor (TCR) signaling which is important for antibody production.
Biological function summary

This isoform plays an important role in immune responses by supporting the activation and differentiation of T cells. It is not part of a larger protein complex itself but interacts with other immune cell surface proteins for effective signaling. CD45RO is particularly important in the modulation of signal transduction through its phosphatase activity which removes phosphate groups from specific tyrosine residues on proteins influencing T cell sensitivity and response to antigens.

Pathways

CD45RO influences the JAK/STAT pathway a critical signaling pathway for cytokine receptor signaling in immune cells. It also plays a role in the TCR signaling pathway which is responsible for T cell activation. Within these pathways CD45RO acts in conjunction with other proteins like Lck and ZAP-70 that are also integral parts of T cell activation and proliferation processes essential for adaptive immune responses.

CD45RO is associated with autoimmune disorders such as rheumatoid arthritis where memory T cells play a role in sustaining inflammation. CD45RO also relates to chronic hepatitis B infection as it affects the disease progression through memory T cell-mediated immune responses. In these contexts CD45RO's interaction with other proteins like HLA-DR which is another immune-related surface protein becomes significant as it helps modulate immune responses that either suppress or exacerbate the disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor (PubMed : 35767951). Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
See full target information PTPRC
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107695 PubMed37822497

2023

Naive T lymphocytes chemotax long distance to CCL21 but not to a source of bioactive S1P.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Garcia-Seyda,Solene Song,Valentine Seveau de Noray,Luc David-Broglio,Christoph Matti,Marc Artinger,Florian Dupuy,Martine Biarnes-Pelicot,Marie-Pierre Valignat,Daniel F Legler,Marc Bajénoff,Olivier Theodoly

Frontiers in immunology 14:1162669 PubMed37207208

2023

Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.

Applications

Unspecified application

Species

Unspecified reactive species

Charles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo Vilar
View all publications
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