Anti-CD45RO antibody [UCHL1]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD45RO antibody [UCHL1] (AB23)

ab23 (1 μg/ml) staining CD45RO in human tonsil.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.1. Slides were peroxidase blocked in 3% H₂O₂ in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with hematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

• WB

Unknown

Western blot - Anti-CD45RO antibody [UCHL1] (AB23)

CD45RO contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-CD45RO antibody [UCHL1] (ab23) at 5 µg/mL

Lane 1:

Human tonsil normal tissue lysate - total protein (<a href='/en-us/products/unavailable/human-tonsil-normal-tissue-lysate-total-protein-ab29615'>ab29615</a>) at 20 µg

Lane 2:

Human thymus tissue lysate - total protein (<a href='/en-us/products/unavailable/human-thymus-tissue-lysate-total-protein-ab30146'>ab30146</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 147 kDa

Observed band size: 180 kDa

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Exposure time: 8min