Anti-CD46 antibody [EPR4014]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human thyroid gland carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 at 1/500 dilution staining CD46 in paraffin-embedded human kidney tissue

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 at 1/500 dilution staining CD46 in paraffin-embedded human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human normal breast tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human normal placenta tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human normal uterus tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD46 antibody [EPR4014] (AB108307)

ab108307 showing positive staining in human breast carcinoma tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-CD46 antibody [EPR4014] (AB108307)

Western blot : Anti-CD46 antibody [EPR4014] (ab108307) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab108307 was shown to bind specifically to CD46. A band was observed at 50-75 kDa in wild-type A549 cell lysates with no signal observed at this size in CD46 knockout cell line. To generate this image, wild-type and CD46 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

CD46 knockout A549 cell lysate at 20 µg

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

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• WB

Unknown

Western blot - Anti-CD46 antibody [EPR4014] (AB108307)

All lanes:

Western blot - Anti-CD46 antibody [EPR4014] (ab108307) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia cell line) cell lysate at 10 µg

Lane 2:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate at 10 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 10 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 44 kDa

false

• WB

Lab

Western blot - Anti-CD46 antibody [EPR4014] (AB108307)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : CD46 knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab108307 observed at 50-70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108307 was shown to specifically react with when CD46 knockout samples were used. Wild-type and CD46 knockout samples were subjected to SDS-PAGE. ab108307 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-CD46 antibody [EPR4014] (ab108307)

Predicted band size: 44 kDa

false

• WB

CiteAb

Western blot - Anti-CD46 antibody [EPR4014] (AB108307)

CD46 western blot using anti-CD46 antibody [EPR4014] ab108307. Publication image and figure legend from Scambi, C., Ugolini, S., et al., 2015, PLoS One, PubMed 25658605.

ab108307 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab108307 please see the product overview.

Immunoblot analysis of MCP (CD46) of skin biopsies from healthy controls and SSc patients.Protein extract from skin biopsies of 8 SSc patients (4 lesional SSc skin and 4 non-lesional SSc skin) and 8 healthy subjects were analyzed by immunoblot using a specific antibody. Results of a representative experiment of four are shown. Data are expressed as an optical density ratio of MCP (CD46) and β-tubulin.

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